50795-28-3Relevant academic research and scientific papers
Analysis of oxidative cytosine products in DNA exposed to ionizing radiation
Wagner, J. R.
, p. 1280 - 1286 (2007/10/02)
From ionizing radiation studies, we have previously characterized a large number of modifications of cytosine and 2'-deoxycytidine which arise by way of hydroxyl radical or base radical cation reactions.However, it remains a great challenge today to detect and quantitate these lesions in DNA.In the present work, I have developed a method for the analysis of six oxidation products of cytosine in DNA using HF acid hydrolysis and gas chromatography/mass spectrometry with isotopic dilution.The analysis of gamma-irradiated DNA indicated that uracil 5,6-glycols 5-hydroxyuracil and 5-hydroxyhydantoin are the major stable decomposition products of cytosine residues, whereas alloxane and 1-carbamoyl-2-oxo-4,5-dihydroxy-imidazolidine are formed in comparatively lower yields.These results suggest that the major pathway of OH radical induced decomposition of cytosine in DNA involves initial deamination of intermediate 5-hydroxy-6-hydroperoxides. - Keywords: Oxidative DNA damage, mass spectrometry, isotopic dilution, mutagenesis.
