511534-93-3Relevant academic research and scientific papers
PNA monomers fully compatible with standard Fmoc-based solid-phase synthesis of pseudocomplementary PNA
Sugiyama, Toru,Hasegawa, Genki,Niikura, Chie,Kuwata, Keiko,Imamura, Yasutada,Demizu, Yosuke,Kurihara, Masaaki,Kittaka, Atsushi
, p. 3337 - 3341 (2017)
Here we report the synthesis of new PNA monomers for pseudocomplementary PNA (pcPNA) that are fully compatible with standard Fmoc chemistry. The thiocarbonyl group of the 2-thiouracil (sU) monomer was protected with the 4-methoxy-2-methybenzyl group (MMPM), while the exocyclic amino groups of diaminopurine (D) were protected with Boc groups. The newly synthesized monomers were incorporated into a 10-mer PNA oligomer using standard Fmoc chemistry for solid-phase synthesis. Oligomerization proceeded smoothly and the HPLC and MALDI-TOF MS analyses indicated that there was no remaining MMPM on the sU nucleobase. The new PNA monomers reported here would facilitate a wide range of applications, such as antigene PNAs and DNA nanotechnologies.
