52483-84-8 Usage
Uses
Used in Biochemical Research:
4-Nitrophenyl phosphate bis(cyclohexylammonium) salt is used as a substrate in biochemical research for the detection of alkaline phosphatase activity. Its ability to solubilize in aqueous solutions allows for its use in a wide range of assays and experiments.
Used in Colorimetric Assays:
In colorimetric assays, 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt is used as an indicator to measure alkaline phosphatase activity in biological samples. The release of 4-nitrophenol upon enzymatic hydrolysis results in a color change, allowing for the quantification of enzyme activity.
Used in Enzyme-Linked Immunosorbent Assays (ELISA):
4-Nitrophenyl phosphate bis(cyclohexylammonium) salt is employed as a detection agent in ELISA, where it serves as a substrate for alkaline phosphatase-conjugated antibodies. The enzymatic conversion of the substrate to 4-nitrophenol provides a colorimetric signal that can be measured and correlated with the presence of specific antigens in the sample.
Used in Medical Diagnostic Tests:
4-Nitrophenyl phosphate bis(cyclohexylammonium) salt is utilized in the development of medical diagnostic tests, particularly those that involve the detection and quantification of alkaline phosphatase activity. Its use in these tests aids in the diagnosis and monitoring of various diseases and conditions.
Used in Laboratory Research for Enzyme Kinetics and Protein Function:
In laboratory research, 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt is used to study enzyme kinetics and protein function. Its role as a substrate for alkaline phosphatase allows researchers to investigate the enzyme's activity, mechanism, and interactions with other molecules, contributing to a deeper understanding of biological processes and potential therapeutic targets.
Check Digit Verification of cas no
The CAS Registry Mumber 52483-84-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 5,2,4,8 and 3 respectively; the second part has 2 digits, 8 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 52483-84:
(7*5)+(6*2)+(5*4)+(4*8)+(3*3)+(2*8)+(1*4)=128
128 % 10 = 8
So 52483-84-8 is a valid CAS Registry Number.
InChI:InChI=1/C6H6NO6P.2C6H13N.H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6;/h1-4H,(H2,10,11,12);2*6H,1-5,7H2;1H2
52483-84-8Relevant academic research and scientific papers
Bis phosphochloridate, a new phosphorylating agent
Beld, A.,Claesen, C. A. A.,Roersma, E. S.,Schippers, W. J. M.,Keizer, L. M.,Tesser, G. I.
, p. 196 - 202 (2007/10/02)
This communication describes the application of the 2-(methylsulfonyl)ethyl group (Mse) as a base-labile protecting group for the two hydroxyl functions in phosphoric acid monoesters.Due to its perfect stability towards acids, the Mse group can function a
