5282-89-3Relevant articles and documents
Aptamer-based enantioselective competitive binding assay for the trace enantiomer detection
Ruta, Josephine,Ravelet, Corinne,Baussanne, Isabelle,Decout, Jean-Luc,Peyrin, Eric
, p. 4716 - 4719 (2007)
The development of highly enantioselective assays and sensors has received much attention for the determination of enantiomeric impurities at a low level. For chiral compounds, the efficient monitoring of the in selection procedure has allowed the isolation of nucleic acid aptamers which are able to strongly discriminate the target enantiomers. In this paper, we demonstrated for the first time that an aptamer can be successfully used to design a highly enantioselective tool for the trace enantiomer detection. The aptamer-based stereoselective assay was developed using an affinity capillary electrophoresis-based competitive, homogeneous format and an on-capillary mixing approach. Detection of as low as 0.01% of the minor enantiomer in a nonracemic mixture can be achieved, in a short analysis time (5 min).
Powerful probes for glycosidases: Novel, fluorescently tagged glycosidase inhibitors
Hermetter, Albin,Scholze, Hubert,Stuetz, Arnold E.,Withers, Stephen G.,Wrodnigg, Tanja M.
, p. 1339 - 1342 (2001)
1-Amino-1,2,5-trideoxy-2,5-imino-D-mannitol was fluorescently tagged by reaction with dansyl chloride at N-1 or by attachment of a dansyl amide bearing spacer to this centre. Compounds obtained are highly potent inhibitors of β-glucosidase exhibiting Ki values in the single figure nanomolar range. The 1-N-dansyl substituted inhibitor was successfully exploited for binding studies with β-glucosidase from Agrobacterium sp. employing fluorescence spectrometric methods.
Synthesis and photophysical properties of fluorophore-labeled abscisic acid
Hou,Abrams,Balsevich,Irvine,Norstrom,Sikorski,Sinha,Steer
, p. 963 - 974 (2000)
The 8'-benzophenone, 8'-dansylhydrazone, 3'-S-(2-ethyldansylamide), and 3'-S-acetamidofluorescein derivatives of the plant hormone abscisic acid (ABA) have been synthesized for use in photoaffinity labeling (the benzophenone derivative) or fluorescence pr
Structure-guided synthesis of a protein-based fluorescent sensor for alkyl halides
Kang, Myeong-Gyun,Lee, Hakbong,Kim, Beom Ho,Dunbayev, Yerkin,Seo, Jeong Kon,Lee, Changwook,Rhee, Hyun-Woo
supporting information, p. 9226 - 9229 (2017/08/21)
Alkyl halides are potentially mutagenic carcinogens. However, no efficient fluorescent sensor for alkyl halide detection in human-derived samples has been developed to date. Herein, we report a new protein-based fluorescent sensor for alkyl halides. Analysis of the HaloTag holo-crystal structure with its covalently attached ligand revealed an unexpected cavity, allowing for the design of a new fluorogenic ligand. This ligand showed the highest fluorescence response (300-fold) and fastest binding kinetics (t1/2 150 s) to a HaloTag mutant (M175P) protein. This protein-based sensor system was effectively used to detect alkyl halides in human serum and monitor real-time protein alkylation.
A novel synthetic strategy for monosubstituted cyclodextrin derivatives
Di Fabio, Giovanni,Malgieri, Gaetano,Isernia, Carla,D'Onofrio, Jennifer,Gaglione, Maria,Messere, Anna,Zarrelli, Armando,De Napoli, Lorenzo
supporting information; experimental part, p. 3875 - 3877 (2012/05/20)
A first solid phase approach to obtain monosubstituted CD conjugates to different labels has been developed. A new solid support has been designed to get a variety of C-6 monofunctionalized CDs (α, β, MeβCD and HPβCD) covalently linked through a phosphodi