53157-48-5Relevant articles and documents
Structure-activity relationship of serotonin derived tocopherol lipids
Muripiti, Venkanna,Mujahid, Thasneem Yoosuf,Boddeda, Venkata Harsha Vardhan,Tiwari, Shrish,Marepally, Srujan Kumar,Patri, Srilakshmi V.,Gopal, Vijaya
, p. 134 - 148 (2019)
Tocopherol-based lipids are widely used for nucleic acid delivery. Using tocopherol molecules, we designed and synthesized 5-HT functionalized lipids by tethering 5-hydroxytryptamine (5-HT), a small molecule ligand as the head group to a natural amphiphilic molecule namely α-tocopherol (Vitamin E). This is with the aim of delivering nucleic acids specifically into cells expressing the serotonin receptors (5-hydroxytryptamine[5-HT]) which are abundant in the central nervous system. In order to achieve target recognition, we adopted an approach wherein two structurally different lipid molecules having serotonin as the head group was conjugated to tocopherol via different linkers thus generating lipids with either free –NH2 or –OH moiety. The corresponding lipids designated as Lipid A (Tocopheryl carbonate serotonin-NH2) and Lipid B (Tocopheryl 2-hydroxy propyl ammonium serotonin-OH), were formulated with co-lipids 1,2-dioleoyl-sn-glycero-3-phosphatidyl-ethanolamine (DOPE) and 1,2-dioleoyl-sn-glycero-sn-3-phosphatidylcholine (DOPC) and evaluated for their ability to deliver plasmid DNA through reporter gene expression assays in vitro. Furthermore, the physicochemical characteristics and cellular interactions of the formulations were examined using serotonin-receptor enriched cells in order to distinguish the structural and functional attributes of both lipids. Cell-based gene expression studies reveal that in comparison to Lipid A, a formulation of Lipid B prepared with DOPE as the co-lipid, contributes to efficient uptake leading to significant enhancement in transfection. Specific interactions explored by molecular docking studies suggests the role of the hydroxyl moiety and the enantiospecific significance of serotonin- conjugated tocopherol lipids in recognizing these receptors thus signifying a promising lipid-based approach to target the serotonin receptors in the central nervous system.
Method for preparing 5-hydroxytryptamine hydrochloride by utilizing enzymatic fermentation liquor containing 5-hydroxytryptamine
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Paragraph 0009; 0032-0034; 0038-0040; 0044-0046, (2021/02/13)
The invention discloses a method for preparing 5-hydroxytryptamine hydrochloride from 5-hydroxytryptamine-containing enzymatic fermentation liquor, and relates to the technical field of 5-hydroxytryptamine extraction. The method comprises the following steps: concentrating 5-hydroxytryptamine-containing enzymatic fermentation liquor under reduced pressure, mixing the concentrated 5-hydroxytryptamine-containing enzymatic fermentation liquor with an organic solvent, sequentially dropwise adding triethylamine and BOC anhydride at low temperature, heating to 20-25 DEG C after dropwise adding is finished, reacting to obtain an N-BOC-5-hydroxytryptamine reaction solution, adding ethyl acetate, and extracting to obtain an ethyl acetate organic phase; adding ethyl acetate containing hydrochloric acid into the ethyl acetate organic phase for reaction, and performing suction filtration after the reaction is finished to obtain a 5-hydroxytryptamine hydrochloride crude product; and further purifying the obtained 5-hydroxytryptamine hydrochloride crude product to obtain 5-hydroxytryptamine hydrochloride. According t o the invention, high-purity 5-hydroxytryptamine hydrochloride is prepared by optimizing the method for purifying 5-hydroxytryptamine in an enzymatic fermentation liquor, so that the current situation that 5-hydroxytryptamine hydrochloride is difficult to prepare by simply extracting serotonin in the enzymatic fermentation liquor is changed.
Nanosensor for Sensitive Detection of the New Psychedelic Drug 25I-NBOMe
Alfonso, María,Díaz de Gre?u, Borja,Garrido, Eva,Gavi?a, Pablo,Lozano-Torres, Beatriz,Marcos, M. Dolores,Martínez-Má?ez, Ramón,Parra, Margarita,Sancenón, Félix
supporting information, (2020/02/27)
This work reports the synthesis, characterization, and sensing behavior of a hybrid nanodevice for the detection of the potent abuse drug 25I-NBOMe. The system is based on mesoporous silica nanoparticles, loaded with a fluorescent dye, functionalized with
