53403-07-9Relevant academic research and scientific papers
A novel low-molecular-mass pumpkin polysaccharide: Structural characterization, antioxidant activity, and hypoglycemic potential
Huang, Linlin,Li, Fei,Li, Quanhong,Liang, Li,Wei, Yunlu,Yu, Guoyong
, (2020/10/02)
The novel natural low-molecular-mass polysaccharide (SLWPP-3) from pumpkin (Cucurbia moschata) was separated from the waste supernatant after macromolecular polysaccharide production and purified using a DEAE cellulose-52 column and gel-filtration chromatography. Chemical and instrumental studies revealed that SLWPP-3 with a molecular mass of 3.5 kDa was composed of rhamnose, glucose, arabinose, galactose and uronic acid with a weight ratio of 1: 1: 4: 6: 15, and primarily contained →3,6)-β-D-Galp-(1→, →4)-α-GalpA-(1→(OMe), →4)-α-GalpA-(1→, →2,4)-α-D-Rhap-(1→, →3)-β-D-Galp-(1→, →4)-α-D-Glcp, and →4)-β-D-Galp residues in the backbone. The branch chain passes were connected to the main chain through the O-4 atom of glucose and O-3 atom of arabinose. Physiologically, the ability of SLWPP-3 to inhibit carbohydrate-digesting enzymes and DPPH and ABTS radicals, as well as protect pancreatic β cells from oxidative damage by decreasing MDA levels and increasing SOD activities, was confirmed. The findings elucidated the structural types of pumpkin polysaccharides and revealed a potential adjuvant natural product with hypoglycemic effects.
Isolation, purification and structural characterization of a water-soluble polysaccharide HM41 from Halenia elliptica D. Don
Liu, Chun-Lan,Li, Yang,Xu, Gui-Yun,Li, Ya-Shuang
, p. 979 - 983 (2016/06/13)
A water-soluble polysaccharide, HM41, was obtained from Halenia elliptica D. Don by acidic ethanol fractionation and gel filtration. Its homogeneity was confirmed by chromatography using multiple systems. HM41 was composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal), glucose (Glc) with a molar ratio of 1.0:5.5:1.8:3.0:9.4:21. The average molecular weight of HM41 was approximately 1.17 × 104. Periodate oxidation, Smith degradation, methylation and GC, IR, NMR, XRD, GC-MS analysis were used for the structural analysis of HM41. Its main chain was composed mainly of β-(1 → 4)Gal, β-(1 → 4)Glc and β-(1 → 6)Glc. β-(1 → 4)Gal were substituted at 6-O and on average there were 14 branches among 23 main chain residues; (1 → 4)Glc had no branch; (1 → 6)Glc were substituted at 3-O and on average there were 9 branches among 14 main chain residues. The side chain was composed of (1 → 3,6)-Rha, (1 → 4)/(1 → 5)-Ara, (1 → 4)/(1 → 5)-Xyl, (1 → 4,6)-Man and (1 → 2)-Glc. The terminal residue was composed of Ara, Xyl, Man, Gal, and Glc. Then, we demonstrated that HM and HM41 had strong scavenging activities in vitro hydroxyl. Overall, HM and HM41 may have potential applications in the antioxidants for medical and food industry.
Isolation and chemical characterization of a glucogalactomannan of the medicinal mushroom Cordyceps militaris
Smiderle, Fhernanda R.,Sassaki, Guilherme L.,Van Griensven, Leo J.L.D.,Iacomini, Marcello
, p. 74 - 80 (2013/10/08)
Cordyceps militaris dried fruiting bodies were extracted with 5% KOH solution. The extract was purified by freeze-thawing treatment, and dialysis (100 kDa), giving rise to a homogeneous polysaccharide (Mw 23,000 Da). Its monosaccharide composition was mannose (56.7%), galactose (34.5%), and glucose (8.8%). The anomeric configurations were determined by their coupling constants. A complex polysaccharide was identified by NMR and methylation analysis. The HSQC spectrum showed signals at δ 107.7/5.06 and 106.1/5.14; 105.9/5.12 relative to β-D-Galf, and O-2-substituted β-D-Galf units, respectively. The sign at δ 104.4/5.21 corresponded to α-D-Galf. Other signals corresponded to α-D-Manp O-6- and O-2-substituted (δ 100.2/4.94; 100.5/5.27; 100.6/5.23; 100.7/5.16), and δ-D-Manp 2,6-di-O-substituted (from δ 99.3 to 99.9). The main linkages, confirmed by methylation analysis, showed the derivatives: 2,3,4-Me3-Manp (11.9%) and 3,4,6-Me3-Manp (28.6%). The branches were (1 → 6)-linked-α-D-Manp or (1 → 2)-linked-β-D-Galf, terminating with β-D-Galf, α-D-Galf, α-D-Galp, or α-D-Manp. 42.7% of the partially hydrolyzed product consisted of 3,4,6-Me3-Manp, suggesting a (1 → 2)-linked backbone.
A facile method for the synthesis of partially O-methylated alditol acetate standards for GC-MS analysis of galactofuranose-containing structures
He, Jian-Yu,Guo, Yu-Na,Zhang, Ling-Ling,Huang, Lin-Hong
, p. 18 - 20 (2013/10/21)
Mixtures of partially O-methylated alditol acetate standards of galactofuranose were synthesized rapidly. Methyl galactofuranosides were obtained with a yield of 79.9% within 4 h under optimized reaction conditions. Methylation of methyl glycosides was carried out in the presence of BaO/Ba(OH)2·8H2O, giving rise to mixtures of partially methylated glycosides. The batch containing the most diverse structures of methyl ethers was converted into partially O-methylated alditol acetates (PMAAs) and then subjected to GC-MS. These PMAAs could be used as GC-MS standards for simultaneous identification of galactofuranose units with diverse linkages in complex carbohydrates.
Structural characterization and protective effect against murine sepsis of fucogalactans from Agaricus bisporus and Lactarius rufus
Ruthes, Andrea C.,Rattmann, Yanna D.,Carbonero, Elaine R.,Gorin, Philip A.J.,Iacomini, Marcello
experimental part, p. 1620 - 1627 (2012/04/11)
Fucogalactans from edible Agaricus bisporus (RFP-Ab) and wild Lactarius rufus (RFP-Lr) mushrooms were obtained on aqueous extraction followed by purification. RFP-Ab had Mw 43.8 × 104 g mol -1 and RFP-Lr Mw 1.4
Chemical analysis of an immunoenhancing water-soluble polysaccharide of an edible mushroom, Pleurotus florida blue variant
Dey, Biswajit,Bhunia, Sanjoy K.,Maity, Kankan K.,Patra, Sukesh,Mandal, Soumitra,Maiti, Swatilekha,Maiti, Tapas K.,Sikdar, Samir R.,Islam, Syed S.
body text, p. 2736 - 2741 (2011/02/21)
An immunoenhancing polysaccharide isolated from the aqueous extract of the fruit bodies of the mushroom, Pleurotus florida blue variant, was found to consist of d-glucose and d-galactose in a molar ratio of nearly 5:1. On the basis of sugar analysis, methylation analysis, periodate oxidation, Smith degradation, and NMR studies (1H, 13C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of the polysaccharide was established as: The molecule activated macrophages, splenocytes, and thymocytes.
Furostan saponin and GC-MS study toward ascertaining the point of linkage between sugars moieties of spirostan saponin from the rhizomes of Agapanthus africanus (Linn.)
Singh,Verma
experimental part, p. 999 - 1003 (2011/04/26)
The linkage between the sugars moieties of antifungal spirostan saponin, (25R)-5α-spirost-7-en-2α,3β,5α-triol-3-O-{-O-α-L- rhamnopyranosyl-(1→2)-O-[β-D-galactopyranosyl-(1→3)] -β-D-glucopyranoside} of Agapanthus africanus (Linn.) have been determined by the analysis of GC-MS spectra of alditol acetates of the partially methylated sugars and the structures of the spirostan saponin and furostan saponin, 22-O-methyl-26-O-β-D-glucopyranosyl-(25R)-5α-furostan-2α, 3β,5α,9α,22ζ,26-hexitol-3-O-{-O-α-L-rhamnopyranosyl- (1→2)-O-[β-D-galactopyranosyl-(1→3)]-β-D-glucopyranoside} isolated from the rhizomes of the A. africanus have been established by various spectral evidences. This is the first report of furostan saponin in A. africanus rhizomes as well as first account of the use of GC-MS technique to ascertain the linkage between the saccharide moieties of the active constituent of the plant.
Triterpenoid Glycosides of Corchorus acutangulus Lam.
Mahato, Shashi B.,Pal, Bikas C.
, p. 629 - 634 (2007/10/02)
Four new triterpenoid glycosides, corchorusins A,B,C, and D, isolated from the aerial part of Corchorus acutangulus Lam. were respectively defined as longispinogenin 3-O-β-D-galactopyranoside (1), saikogenin F 3-O-β-D-galactopyranoside (12), 23-hydroxylongispinogenin 3-O-β-D-galactopyranoside (6), and saikogenin E 3-O-β-D-glucopyranosyl(12)-β-D-galactopyranoside (15) based on their spectral properties and some chemical transformations.
THE STRUCTURAL ELUCIDATION OF THE CAPSULAR POLYSACCHARIDE OF Klebsiella K68
Dutton, Guy G. S.,Parolis, Haralambos,Parolis, Lesley A. S.
, p. 249 - 260 (2007/10/02)
The structure of the capsular polysaccharide isolated from Klebsiella K68 bacteria has been elucidated by both chemical and spectroscopic methods.The structure is of the "3+1" type, similar in pattern to the structures of the polysaccharides of Klebsiella K11 and K57, having a single branch point on the uronic acid.The polysaccharide is shown to consist of the following repeating unit:
