536725-66-3

Upstream product

• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 16658-10-9 2',3'-O-(methoxymethylidene)adenosine 

Downstream Products

• 536725-68-5 dithiophosphoric acid O-[6-(6-{[bis-(4-methoxy-phenyl)-phenyl-methyl]-amino}-purin-9-yl)-2-methoxy-tetrahydro-furo[3,4-d][1,3]dioxol-4-ylmethyl] ester S,S'-diphenyl ester 

Relevant academic research and scientific papers

Synthesis of TMG-capped RNA-DNA chimeric oligonucleotides

This paper deals with the synthesis of m32,2,7G5′pppAmpUmpApd(CpTpTpApCpCpT), a 5′-TMG-capped RNA-DNA chimeric oligonucleotide, which is expected to be conveyed to the nucleus by snurportin 1, a nuclear-transport protein. This 5′-TMG-capped RNA-DNA chimeric molecule was synthesized by the enzymatic condensation of m32,2,7G5′pppAmpUmpA with A5′ppd(CpTpTpApCpCpT) in the presence of RNA ligase. An inherent serious side reaction was disclosed in the 5′-adenylation of d(CpTpTpApCpCpT) on the solid support, but the use of an active ester intermediate as the pA donor gave an improved result.