Tetrahedron Letters p. 1703 - 1707 (2003)
Update date:2022-08-03
Topics: Synthesis Experimental
Sekine, Mitsuo
Ushioda, Masatoshi
Wada, Takeshi
Seio, Kohji
This paper deals with the synthesis of m32,2,7G5′pppAmpUmpApd(CpTpTpApCpCpT), a 5′-TMG-capped RNA-DNA chimeric oligonucleotide, which is expected to be conveyed to the nucleus by snurportin 1, a nuclear-transport protein. This 5′-TMG-capped RNA-DNA chimeric molecule was synthesized by the enzymatic condensation of m32,2,7G5′pppAmpUmpA with A5′ppd(CpTpTpApCpCpT) in the presence of RNA ligase. An inherent serious side reaction was disclosed in the 5′-adenylation of d(CpTpTpApCpCpT) on the solid support, but the use of an active ester intermediate as the pA donor gave an improved result.
View MoreXi'an Unique Electronic and Chemical Co., Ltd.
Contact:+86-029-88238008
Address:1703# B BUILDING WEST ELECTRONIC ZONE, XI'AN, CHINA
Goldwills Pharmaceuticals Co., Ltd.
Contact:0916-2237889 13991621155
Address:North Suburb of Hanzhong city, Shaanxi Province
Taixing Shenfeng Chemical Co., Ltd.
Contact:+86-523-87117033, 87117666
Address:4# Yinsanlu, Huangqiao town, Taixing, Jiangsu, China.
ChangZhou XiaQing Chemical Co., Ltd.
Contact:+86-519-88721665
Address:3# Hengluo Road, Henglin Town, Changzhou City, Jiangsu Province,China
Shanghai Pengkai Chemical Co.,Ltd
Contact:+86-21-60526671
Address:No.4226 Duzhuang Rd Minhang District Shanghai China
Doi:10.1021/jo00894a012
(1975)Doi:10.1021/jo00895a022
(1975)Doi:10.1002/mrc.1270060614
(1974)Doi:10.1016/0040-4020(66)80113-8
(1966)Doi:10.1007/BF00913850
(1952)Doi:10.1016/S0040-4039(03)00101-1
(2003)