537677-37-5

Upstream product

• 112883-29-1 N-Fmoc-Tyr-OH 
• 208655-83-8 allyl 2-(((9H-fluoren-9-yl)methoxy)carbonylamino)-3-(4-tert-butoxyphenyl)propanoate 
• 143674-78-6 (S)-allyl 2-(((9H-fluoren-9-yl)methoxy)carbonylamino)-3-(4-hydroxyphenyl)propanoate 
• 71989-38-3 Fmoc-Tyr(tBu)-OH 
• 537677-38-6 N^α-(9-fluorenylmethoxycarbonyl)tyrosine 2-chlorotrityl ester 
• 208655-84-9 2',3'-di-O-acetyluridine-5'-O-yl(2-cyanoethoxy)(diisopropylamino)phosphane 
• 29108-90-5 2',3'-di-O-acetyl-uridine 
• 537677-39-7 (S)-3-(4-{(2-Cyano-ethoxy)-[(2R,3R,4R,5R)-3,4-diacetoxy-5-(2,4-dioxo-3,4-dihydro-2H-pyrimidin-1-yl)-tetrahydro-furan-2-ylmethoxy]-phosphoryloxy}-phenyl)-2-(9H-fluoren-9-ylmethoxycarbonylamino)-propionic acid (2-chloro-phenyl)-diphenyl-methyl ester 
• 208655-85-0 (S)-3-(4-{(2-Cyano-ethoxy)-[(2R,3R,4R,5R)-3,4-diacetoxy-5-(2,4-dioxo-3,4-dihydro-2H-pyrimidin-1-yl)-tetrahydro-furan-2-ylmethoxy]-phosphoryloxy}-phenyl)-2-(9H-fluoren-9-ylmethoxycarbonylamino)-propionic acid allyl ester 

Downstream Products

• 208655-87-2 C₃₇H₄₈N₇O₂₀P 

Relevant academic research and scientific papers

A general synthetic method toward uridylylated picornavirus VPg proteins

Small proteins called viral protein genome-linked (VPg), attached to the 5′-end of the viral RNA genome are found as common structure in the large family of picornaviruses. The replication of these viruses is primed by this VPg protein linked to a single uridylyl residue. We report a general procedure to obtain such nucleoproteins employing a pre-uridylylated tyrosine building block in an on-line solid phase-based approach. Copyright 2013 European Peptide Society and John Wiley & Sons, Ltd. The replication of picornaviruses is primed by a small so-called VPg protein linked to a uridylyl residue. We report a general procedure to obtain such nucleoproteins employing a pre-uridylylated tyrosine building block in an on-line solid phase based approach.

Stepwise solid phase synthesis of uridylylated viral genome-linked peptides using uridylylated amino acid building blocks

The uridylylated amino acid building blocks 2-cyanoethyl-(Nα-9-fluorenylmethoxy-carbonyl-tyrosin-4-yl)- (2′,3′-di-O-acetyluridin-5′-yl) phosphate and 2-chlorophenyl-(Nα-fluorenyl-methoxycarbonyl-serin-3-yl)- (2′,3′-di-O-acetyluridin-5′-yl) phosphate have been used successfully in an on-line SPPS of the VPgpU from the polio, coxsackie and cowpea mosaic virus.

Synthesis of a nucleopeptide fragment from poliovirus genome

Condensation of a properly protected uridylylated nucleopeptide with a heptadecapeptide gives after one-step deprotection the target nucleopeptide H-Gly-Ala-Tyr(5pU)-Thr-Gly-Leu-Pro-Asn-Lys-Lys-Pro-Asn-Val-Pro-Thr-Ile-Arg- Thr-Ala-Lys-Val-Gln-OH.