53989-91-6Relevant academic research and scientific papers
Labeled nucleosides and method for their preparation
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, (2008/06/13)
The invention relates to a method for the chemical preparation of labeled nucleosides via an oxidative-ring-opening step and a reductive-ring-closure step starting from a labeled D-glucose nucleoside resulting in the corresponding D-ribose-nucleoside by removing the carbon atom 3 on the glucose and comprising the steps of:1. a protection of the sugar ring hydroxyl groups,2. a sugar-base condensation, whereby the base is a purine or a pyrimidine,3. a deprotection of the sugar ring hydroxyl groups,4. an oxidative-ring-opening and removal of the carbon atom 3, and5. a reductive-ring-closure resulting in labeled D-ribose-nucleoside.The invention further relates to a compound obtainable via the method according to the invention having the formula selected from [13C,15N] labeled 6'-O-mono- or 6'-O-bis-C1-C6-alkyloxytrityl-D-glycopyranosyl nucleosides, or [13C,15N] labeled tetra-O-acetyl-D-glycopyranosyl nucleosides or [13C,15N] labeled glycopyranosyl nucleosides.
