5565-36-6Relevant articles and documents
Anionic Conjugated Polyelectrolytes for FRET-based Imaging of Cellular Membrane Potential
Delehanty, James B.,Jeong, Ji-Eun,Le, Van Sang,Nag, Okhil K.,Oh, Eunkeu,Woo, Han Young
, (2020)
We report a F?rster resonance energy transfer (FRET)-based imaging ensemble for the visualization of membrane potential in living cells. A water-soluble poly(fluorene-cophenylene) conjugated polyelectrolyte (FsPFc10) serves as a FRET donor to a voltage-sensitive dye acceptor (FluoVolt?). We observe FRET between FsPFc10 and FluoVolt?, where the enhancement in FRET-sensitized emission from FluoVolt? is measured at various donor/acceptor ratios. At a donor/acceptor ratio of 1, the excitation of FluoVolt? in a FRET configuration results in a three-fold enhancement in its fluorescence emission (compared to when it is excited directly). FsPFc10 efficiently labels the plasma membrane of HEK 293T/17 cells and remains resident with minimal cellular internalization for ~ 1.5 h. The successful plasma membrane-associated colabeling of the cells with the FsPFc10-FluoVolt? donor-acceptor pair is confirmed by dual-channel confocal imaging. Importantly, cells labeled with FsPFc10 show excellent cellular viability with no adverse effect on cell membrane depolarization. During depolarization of membrane potential, HEK 293T/17 cells labeled with the donor-acceptor FRET pair exhibit a greater fluorescence response in FluoVolt? emission relative to when FluoVolt? is used as the sole imaging probe. These results demonstrate the conjugated polyelectrolyte to be a new class of membrane labeling fluorophore for use in voltage sensing schemes.
Rhodium(I)-catalyzed borylation of nitriles through the cleavage of carbon-cyano bonds
Tobisu, Mamoru,Kinuta, Hirotaka,Kita, Yusuke,Remond, Emmanuelle,Chatani, Naoto
, p. 115 - 118 (2012/03/07)
The reaction of aryl cyanides with diboron in the presence of a rhodium/Xantphos catalyst and DABCO affords arylboronic esters via carbon-cyano bond cleavage. This unprecedented mode of reactivity for a borylrhodium species allows the regioselective intro
Synthesis, optical, and electronic properties of soluble poly-p-phenylene oligomers as models for molecular wires
Banerjee, Moloy,Shukla, Ruchi,Rathore, Rajendra
supporting information; experimental part, p. 1780 - 1786 (2009/07/25)
A homologous series of soluble poly-p-phenylenes containing up to eight phenylene moieties(pp2-pp8)with branched iso-alkyl(or bis-n-alkylmethyl)groups has been synthesized and the structure-property relationship with regards to various optical and electronic properties established. All electronic and optoelectronic properties of poly-p-phenylenes followed a 1/n relationship(where n is number of phenylene moieties)with the increasing number of phenylene moieties. The low-energy electronic transition of the pp2-PP7 cation radicals, generated either by laser-flash photolysis or by chemical oxidation,varied as well according to the inverse(1/n)relationship. The observed inverse relationship of all measured electronic and optoelectronic prope rties against the increasing number of phenylene units in various ppn's, as well as X-ray crystallography of both neutral and a cation-radical salt of a representative tetra-p-phenylene oligomer allows us to demonstrate that the effective conjugation length in poly-p-phenylenes is, in part, controlled by the increasing number of interactions of ortho hydrogens which may prevent simultaneous planarization of the continuous arrays of a large number of phenylene moieties.
