56360-46-4

Basic information

• Product Name: EOSIN B
• Synonyms: 4,5-DIBROMO-2,7-DINITROFLUORESCEIN;4',5'-DIBROMO-2',7'-DINITROFLUORESCEIN DISODIUM SALT;4,5-DIBROMO-2,7-DINITROFLUORESCEIN DISODIUM SALT;ACID RED 91;EOSIN B;EOSIN BA OR BN;EOSIN B DISODIUM SALT;EOSIN BLUE SHADE
• CAS NO: 56360-46-4
• Molecular Formula: C20H8Br2N2O9
• Molecular Weight: 580.1
• EINECS: 208-943-1
• Product Categories: Hematology and Histology;Hematology Stains
• Mol File: 56360-46-4.mol
• Article Data: 1

Chemical Properties

• Melting Point: 275 °C (dec.)(lit.)
• Boiling Point: 663.6 °C at 760 mmHg
• Flash Point: 355.1 °C
• Appearance: Orange/Powder
• Density: g/cm³
• Solubility: spirit: soluble
• PKA: 5.98±0.20(Predicted)
• CAS DataBase Reference: EOSIN B(CAS DataBase Reference)
• NIST Chemistry Reference: EOSIN B(56360-46-4)
• EPA Substance Registry System: EOSIN B(56360-46-4)

Safety Data

• Hazard Codes: Xn
• Statements: 22-21/22
• Safety Statements: 22-24/25-36/37
• WGK Germany: 3
• Hazardous Substances Data: 56360-46-4(Hazardous Substances Data)

Usage

Chemical Properties

orange powder

Definition

ChEBI: A C-nitro compound which consists of a fluorescein skeleton substituted by bromo groups at positions 4 and 5 and nitro groups at positions 2 and 7.

Upstream product

• 596-03-2 4',5'-dibromofluorescein 
• 2321-07-5 fluorescein 
• 108-46-3 recorcinol 
• 2513-33-9 2-(2,4-dihydroxybenzoyl)benzoic acid 
• 85-44-9 phthalic anhydride 
• 15086-94-9 eosin y 

Downstream Products

• 1253821-19-0 ethyl 2-(2',7'-dibromo-6'-hydroxy-4',5'-dinitro-3'-oxo-3H-xanthen-9'-yl)benzoate 

Relevant articles and documents

Novel β-cyclodextrin-eosin conjugates

Eosin B (EoB) and eosin Y (EoY), two xanthene dye derivatives with photosensitizing ability were prepared in high purity through an improved synthetic route. The dyes were grafted to a 6-monoamino-β-cyclodextrin scaffold under mild reaction conditions through a stable amide linkage using the coupling agent 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride. The molecular conjugates, well soluble in aqueous medium, were extensively characterized by 1D and 2D NMR spectroscopy and mass spectrometry. Preliminary spectroscopic investigations showed that the β-cyclodextrin-EoY conjugate retains both the fluorescence properties and the capability to photogenerate singlet oxygen of the unbound chromophore. In contrast, the corresponding β-cyclodextrin-EoB conjugate did not show either relevant emission or photosensitizing activity probably due to aggregation in aqueous medium, which precludes any response to light excitation.