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CAS

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596-03-2

4',5'-DIBROMOFLUORESCEIN is a synthetic, brilliant yellow-orange powder that exhibits unique solubility and fluorescence properties when dissolved in various solvents. It is slightly soluble in water, showing an orange color with weak yellow fluorescence, soluble in ethanol, displaying an orange color with green-yellow fluorescence, and soluble in acetone, presenting a peach blossom color with yellow fluorescence. Additionally, it is soluble in Tetrahydrofurfuryl alcohol. When in concentrated sulfuric acid, it appears as a red light yellow color, which turns to yellow brown upon dilution, accompanied by the formation of an orange precipitate. The dye's aqueous solution turns red upon the addition of sodium hydroxide. It demonstrates good dispersibility in glycerol and liquid paraffin.

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  • 596-03-2 Structure

  • Basic information

    1. Product Name: 4',5'-DIBROMOFLUORESCEIN
    2. Synonyms: SOLVENT RED 72;4’,5’-dibromo-fluorescei;4’,5’-dibromofluororescein;9’-(9H)xanthen)-3-one,4’,5’-dibromo-3’,6’-dihydroxy-Spiro(isobenzofuran-1(3H);acidorange11;c.i.solventred72;d&corangeno.5;eosinicacid
    3. CAS NO:596-03-2
    4. Molecular Formula: C20H10Br2O5
    5. Molecular Weight: 490.1
    6. EINECS: 209-876-0
    7. Product Categories: N/A
    8. Mol File: 596-03-2.mol

  • Chemical Properties

    1. Melting Point: 270-273 °C(lit.)
    2. Boiling Point: 633.7 °C at 760 mmHg
    3. Flash Point: 337 °C
    4. Appearance: Orange Powder.
    5. Density: 1.6743 (rough estimate)
    6. Vapor Pressure: 0Pa at 25℃
    7. Refractive Index: 1.5010 (estimate)
    8. Storage Temp.: Sealed in dry,Room Temperature
    9. Solubility: N/A
    10. PKA: 8.14±0.20(Predicted)
    11. Water Solubility: 153.49μg/L at 25℃
    12. Merck: 14,3022
    13. BRN: 57189
    14. CAS DataBase Reference: 4',5'-DIBROMOFLUORESCEIN(CAS DataBase Reference)
    15. NIST Chemistry Reference: 4',5'-DIBROMOFLUORESCEIN(596-03-2)
    16. EPA Substance Registry System: 4',5'-DIBROMOFLUORESCEIN(596-03-2)

  • Safety Data

    1. Hazard Codes: Xi,T
    2. Statements: 36-25-36/37/38
    3. Safety Statements: 22-24/25-45-36-26
    4. RIDADR: UN 2811 6.1/PG 3
    5. WGK Germany: 3
    6. RTECS: LM5200000
    7. TSCA: Yes
    8. HazardClass: N/A
    9. PackingGroup: N/A
    10. Hazardous Substances Data: 596-03-2(Hazardous Substances Data)

596-03-2 Usage

Uses

Used in Dyes and Metabolites:
4',5'-DIBROMOFLUORESCEIN is used as a stain in the field of dyes and metabolites, particularly for environmental testing. Its absorption maximum of 450 nm makes it a valuable tool for detecting and analyzing various substances in environmental samples.
Used in Medicines and Cosmetics Coloring:
4',5'-DIBROMOFLUORESCEIN is utilized as a coloring agent in the pharmaceutical and cosmetics industries. Its vibrant color and fluorescence properties contribute to the visual appeal and effectiveness of various products, enhancing their marketability and consumer appeal.
Used in Environmental Testing:
In the environmental sector, 4',5'-DIBROMOFLUORESCEIN is employed as a stain for detecting and analyzing pollutants and other substances in environmental samples. Its unique fluorescence properties allow for the identification and quantification of specific contaminants, aiding in the assessment of environmental health and pollution control efforts.

Preparation

Fluorescein (C.I. Acid Yellow 73, C.I. 45350) in the sodium hydroxide solution two bromination, and??made into a free acid form.

Safety Profile

Poison by ingestion and intravenous routes. An herbicide. When heated to decomposition it emits highly toxic fumes of NOx, CN-, and Br-. See also NITRILES.

Purification Methods

Crystallise it from aqueous 30% EtOH. [Beilstein 19/6 V 462.]

Check Digit Verification of cas no

The CAS Registry Mumber 596-03-2 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 5,9 and 6 respectively; the second part has 2 digits, 0 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 596-03:
(5*5)+(4*9)+(3*6)+(2*0)+(1*3)=82
82 % 10 = 2
So 596-03-2 is a valid CAS Registry Number.

596-03-2 Well-known Company Product Price

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  • Alfa Aesar

  • (A18226)  4',5'-Dibromofluorescein, ca. 95% dye content   

  • 596-03-2

  • 50g

  • 271.0CNY

  • Detail

  • Alfa Aesar

  • (A18226)  4',5'-Dibromofluorescein, ca. 95% dye content   

  • 596-03-2

  • 250g

  • 940.0CNY

  • Detail

596-03-2SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 12, 2017

Revision Date: Aug 12, 2017

1.Identification

1.1 GHS Product identifier

Product name 4',5'-Dibromofluorescein

1.2 Other means of identification

Product number -
Other names 4',5'-dibromo-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:596-03-2 SDS

596-03-2Relevant articles and documents

3D-Non-destructive Imaging through Heavy-Metal Eosin Salt Contrast Agents

Busse, Madleen,Marciniszyn, Jaroslaw P.,Ferstl, Simone,Kimm, Melanie A.,Pfeiffer, Franz,Gulder, Tanja

supporting information, p. 4561 - 4566 (2021/02/12)

Conventional histology is a destructive technique based on the evaluation of 2D slices of a 3D biopsy. By using 3D X-ray histology these obstacles can be overcome, but their application is still restricted due to the inherently low attenuation properties of soft tissue. In order to solve this problem, the tissue can be stained before X-ray computed tomography imaging (CT) to enhance the soft tissue X-ray contrast. Evaluation of brominated fluorescein salts revealed a mutual influence of the number of bromine atoms and the cations applied on the achieved contrast enhancement. The dibromo fluorescein barium salt turned out to be the ideal X-ray contrast agent, allowing for 3D imaging and subsequent complementing counterstaining applying standard histological techniques.

Novel β-cyclodextrin-eosin conjugates

Benkovics, Gábor,Afonso, Damien,Darcsi, András,Béni, Szabolcs,Conoci, Sabrina,Fenyvesi, éva,Szente, Lajos,Malanga, Milo,Sortino, Salvatore

supporting information, p. 543 - 551 (2017/03/29)

Eosin B (EoB) and eosin Y (EoY), two xanthene dye derivatives with photosensitizing ability were prepared in high purity through an improved synthetic route. The dyes were grafted to a 6-monoamino-β-cyclodextrin scaffold under mild reaction conditions through a stable amide linkage using the coupling agent 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride. The molecular conjugates, well soluble in aqueous medium, were extensively characterized by 1D and 2D NMR spectroscopy and mass spectrometry. Preliminary spectroscopic investigations showed that the β-cyclodextrin-EoY conjugate retains both the fluorescence properties and the capability to photogenerate singlet oxygen of the unbound chromophore. In contrast, the corresponding β-cyclodextrin-EoB conjugate did not show either relevant emission or photosensitizing activity probably due to aggregation in aqueous medium, which precludes any response to light excitation.

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