5725-91-7 Usage
Uses
Used in Pharmaceutical Industry:
Resorufin Ethyl Ether is used as a fluorimetric substrate for cytochrome P450 IAI, which aids in the study of the enzyme's activity and its role in drug metabolism.
Used in Biomedical Research:
Resorufin Ethyl Ether is used as a dealkylase substrate for the microfluorimetric analysis of microsomal cytochrome P-450, allowing researchers to evaluate the enzyme's function and its interactions with various inhibitors.
Used in Enzyme Activity Studies:
Resorufin Ethyl Ether is used as a suitable substrate for studying the dealkylating activity of cytochrome P450 isozymes 1A1 and 1A2, which are crucial in understanding the enzyme's role in drug metabolism and detoxification processes.
Used in Inhibitor Studies:
Resorufin Ethyl Ether is used to study the ethoxyresorufin-O-deethylase (EROD) activity of cytochrome P450 activity (CYP1A1), which helps in evaluating the effectiveness of arylalkyl and alkyl isothiocyanates, and their glutathione, cysteine, and A′-acetylcysteine conjugates as inhibitors.
Used in Cardiovascular Research:
Resorufin Ethyl Ether is used to inhibit vasorelaxant responses to acetylcholine, which can provide insights into the underlying mechanisms of vascular function and potential therapeutic targets for cardiovascular diseases.
Biological Activity
cytochrome p450, family 1, subfamily a, polypeptide 1 is a protein that is encoded by human cyp1a1 gene. the protein is a member of the cytochrome p450 superfamily of enzymes. cyp1a1 is involved in phase i xenobiotic and drug metabolism. cyp1a1 is inhibited by fluoroquinolones and macrolides and induced by aromatic hydrocarbons. 7-ethoxyresorufin is a specific subtrate of cyp1a.
in vitro
7-ethoxyresorufin (2-10 microm), a substrate for cytochrome p450, which binds to the heme moiety of the enzyme, reduced responses to acetylcholine concentration-dependently but not the other agonists indicating an effect on nitric oxide synthesis although neither affected the conversion of l-arginine to l-citrulline [1].
in vivo
activities of cyp1a (7-ethoxyresorufin) in the liver was determined for comparative purposes. in mice, lps depressed cyp2a5 at low doses but not at doses that increased pro-inflammatory cytokines and no serum levels, and depressed cyp1a activity. blockade of proinflammatory cytokines extended down-regulation of cyp2a5 while not affecting lps-induced depression of cyp1a [2].
references
[1] oyekan ao, mcgiff jc, rosencrantz-weiss p, quilley j. relaxant responses of rabbit aorta: influence of cytochrome p450 inhibitors. j pharmacol exp ther. 1994 jan;268(1):262-9.[2] de-oliveira ac, po?a ks, totino pr, paumgartten fj. modulation of cytochrome p450 2a5 activity by lipopolysaccharide: low-dose effects and non-monotonic dose-response relationship. plos one. 2015 jan 30;10(1):e0117842. [3] pelkonen o, pasanen m, kuha h, gachalyi b, kairaluoma m, sotaniemi ea, park ss, friedman fk, gelboin hv. the effect of cigarette smoking on 7-ethoxyresorufin o-deethylase and other monooxygenase activities in human liver: analyses with monoclonal antibodies. br j clin pharmacol. 1986 aug;22(2):125-34.
Check Digit Verification of cas no
The CAS Registry Mumber 5725-91-7 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 5,7,2 and 5 respectively; the second part has 2 digits, 9 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 5725-91:
(6*5)+(5*7)+(4*2)+(3*5)+(2*9)+(1*1)=107
107 % 10 = 7
So 5725-91-7 is a valid CAS Registry Number.
InChI:InChI=1/C14H11NO3/c1-2-17-10-4-6-12-14(8-10)18-13-7-9(16)3-5-11(13)15-12/h3-8H,2H2,1H3
