5845-45-4Relevant academic research and scientific papers
An Improvment of the Anderson Test by the Use of High Performance Liquid Chromatography
Miyazawa, Toshifumi,Yamada, Takashi,Kuwata, Shigeru
, p. 606 - 608 (1988)
The replacement of the N-benzyloxycarbonyl (Z) group in the Anderson peptide (Z-Gly-L/D-Phe-Gly-OEt) by Z-L-Ala and the subsequent separation of the diastereomers of the resulting tetrapeptide by reversed-phase HPLC offer a more convenient version with higher accuracy to the original Anderson test for studying racemization in peptide synthesis.
HMDO-promoted peptide and protein synthesis in ionic liquids
Duan, Jianli,Sun, Yao,Chen, Hao,Qiu, Guofu,Zhou, Haibing,Tang, Ting,Deng, Zixin,Hong, Xuechuan
, p. 7013 - 7022 (2013/08/23)
Hexamethyldisiloxane (HMDO) has been developed to efficiently promote the metal-free direct coupling of an amino function of one cysteine-free peptide or protein and a C-terminal thioester of the second peptide in ionic liquids. The amide-coupling reaction proceeds smoothly under mild conditions to afford the corresponding products in good to excellent yields (63-94%). Peptide couplings were also achieved using in-situ-generated thioesters by the thioesterification of oxo esters.
