An Improvment of the Anderson Test by the Use of High Performance Liquid Chromatography

Article abstract of DOI:10.1246/bcsj.61.606

The replacement of the N-benzyloxycarbonyl (Z) group in the Anderson peptide (Z-Gly-L/D-Phe-Gly-OEt) by Z-L-Ala and the subsequent separation of the diastereomers of the resulting tetrapeptide by reversed-phase HPLC offer a more convenient version with higher accuracy to the original Anderson test for studying racemization in peptide synthesis.