58823-95-3Relevant articles and documents
Continuous-flow reactor-based enzymatic synthesis of phosphorylated compounds on a large scale
Babich, Lara,Hartog, Aloysius F.,Van Der Horst, Michael A.,Wever, Ron
experimental part, p. 6604 - 6609 (2012/07/14)
Acid phosphatase, an enzyme that is able to catalyze the transfer of a phosphate group from cheap pyrophosphate to alcoholic substrates, was covalently immobilized on polymethacrylate beads with an epoxy linker (Immobeads-150 or Sepabeads EC-EP). After immobilization 70 % of the activity was retained and the immobilized enzyme was stable for many months. With the immobilized enzyme we were able to produce and prepare D-glucose-6-phosphate, N-acetyl-D-glucosamine- 6-phosphate, allyl phosphate, dihydroxyacetone phosphate, glycerol-1-phosphate, and inosine-5'-monophosphate from the corresponding primary alcohol on gram scale using either a fed-batch reactor or a continuous-flow packed-bed reactor. Copyright