59-85-8 Usage
Uses
Used in Enzyme Inhibition:
4-CHLOROMERCURIBENZOIC ACID is used as an enzyme inhibitor for targeting enzymes that require unmodified cysteine residues, such as adenylyl cyclase. Its application in this area aids in the study and control of various biological processes and pathways.
Used in Sulfhydryl Reactivity:
As a sulfhydryl reagent, 4-CHLOROMERCURIBENZOIC ACID is used to react with specific residues, such as Histidine 225C, in the NhaA-Na/H Antiporter of Escherichia Coli. This application is crucial for understanding the structure and function of proteins and their role in cellular processes.
Used in Pharmaceutical Research:
4-CHLOROMERCURIBENZOIC ACID's ability to inhibit enzymes and react with specific residues makes it a valuable tool in pharmaceutical research. It can be utilized in the development of new drugs and therapies targeting various diseases and conditions.
Used in Biochemical Studies:
In the field of biochemistry, 4-CHLOROMERCURIBENZOIC ACID is used to study the interactions between enzymes and their substrates or inhibitors. This helps researchers gain insights into the mechanisms of enzyme catalysis and the development of novel bioactive compounds.
Used in Chemical Synthesis:
4-CHLOROMERCURIBENZOIC ACID can also be employed as a starting material or intermediate in the synthesis of various organic compounds, particularly those with potential applications in the pharmaceutical, agrochemical, and materials science industries.
Safety Profile
Poison by
intraperitoneal route. See also MERCURY
COMPOUNDS. When heated to
decomposition it emits very toxic fumes of
Cland Hg.
Purification Methods
Its suspension in water is stirred with enough 1M NaOH to dissolve most of it: a small amount of insoluble matter is removed by centrifugation. The chloromercuribenzoic acid is then precipitated by adding 1M HCl and centrifuged off. The precipitation is repeated twice. Finally, the precipitate is washed three times with distilled water (by centrifugation), then dried in a thin layer under vacuum over P2O5 [Boyer J Am Chem Soc 76 4331 1954]. Chloromethylphosphonic acid dichloride [1983 -26 -2] M 167.4, b 50o/0.5mm, 52 -53(59)o/2mm, 63-65o/3mm, 78 -79o/10mm, 87 -88o/15mm, 102-103o/30mm, d 4 1.638, n D 1.4971. It is fractionally distilled using a short Claisen column and redistilled. The aniline salt has m 199-201o. The 3 1P NMR has a single peak at -38±2 ppm from 85% H3PO4. [Kinnear & Perren J Chem Soc 3437 1952, NMR: van Wazer et al. J Am Chem Soc 78 5715 1956, McConnell et al. J Org Chem 22 462 1957, Beilstein 1 III 2593, 1 IV 3068.]
Check Digit Verification of cas no
The CAS Registry Mumber 59-85-8 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 5 and 9 respectively; the second part has 2 digits, 8 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 59-85:
(4*5)+(3*9)+(2*8)+(1*5)=68
68 % 10 = 8
So 59-85-8 is a valid CAS Registry Number.
InChI:InChI=1/C7H5O2.ClH.Hg/c8-7(9)6-4-2-1-3-5-6;;/h2-5H,(H,8,9);1H;/q;;+1/p-1/rC7H5HgO2.ClH/c8-6-3-1-5(2-4-6)7(9)10;/h1-4H,(H,9,10);1H/q+1;/p-1
