606490-81-7Relevant articles and documents
From Bugs to Bioplastics: Total (+)-Dihydrocarvide Biosynthesis by Engineered Escherichia coli
Ascue Avalos, Gabriel A.,Toogood, Helen S.,Tait, Shirley,Messiha, Hanan L.,Scrutton, Nigel S.
, p. 785 - 792 (2019/01/29)
The monoterpenoid lactone derivative (+)-dihydrocarvide ((+)-DHCD) can be polymerised to form shape-memory polymers. Synthetic biology routes from simple, inexpensive carbon sources are an attractive, alternative route over chemical synthesis from (R)-carvone. We have demonstrated a proof-of-principle in vivo approach for the complete biosynthesis of (+)-DHCD from glucose in Escherichia coli (6.6 mg L?1). The pathway is based on the Mentha spicata route to (R)-carvone, with the addition of an ′ene′-reductase and Baeyer–Villiger cyclohexanone monooxygenase. Co-expression with a limonene synthesis pathway enzyme enables complete biocatalytic production within one microbial chassis. (+)-DHCD was successfully produced by screening multiple homologues of the pathway genes, combined with expression optimisation by selective promoter and/or ribosomal binding-site screening. This study demonstrates the potential application of synthetic biology approaches in the development of truly sustainable and renewable bioplastic monomers.
Oxidation of terpenoids with a cyclohexanone fragment by performic acid
Vydrina,Galkina,Muslukhov,Kravchenko,Ishmuratov
, p. 774 - 775 (2015/02/02)
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Microbial Baeyer-Villiger oxidation of terpenones by recombinant whole-cell biocatalysts - Formation of enantiocomplementary regioisomeric lactones
Ernuchova, Petra,Mihovilovic, Marko D.
, p. 1715 - 1719 (2008/02/10)
Recombinant whole-cell expression systems for Baeyer-Villiger monooxygenases of various bacterial origin were utilized in the regiodivergent biooxidation of cyclic terpenones enabling access to enantio- and regioisomeric lactones on preparative scale. This journal is The Royal Society of Chemistry.
