61043-33-2

Basic information

• Product Name: Z-ALA-ALA-LEU-PNA
• Synonyms: BENZYLOXYCARBONYL-L-ALANYL-L-ALANYL-L-LEUCINE P-NITROANILIDE;CBZ-L-ALA-ALA-LEU-P-NITROANILIDE;CBZ-ALA-ALA-LEU-P-NITROANILIDE;SUBTILISIN A SUBSTRATE II;SUBTILISIN A SUBSTRATE II, COLORIMETRIC;Z-L-ALANYL-L-ALANYL-L-LEUCINE-4-NITROANILIDE;Z-ALA-ALA-LEU-PNA;N-cbz-ala-ala-leu P-nitroanilide
• CAS NO: 61043-33-2
• Molecular Formula: C₂₆H₃₃N₅O₇
• Molecular Weight: 527.57
• Product Categories: Peptide
• Mol File: 61043-33-2.mol
• Article Data: 7

Chemical Properties

• Appearance: /
• Refractive Index: 1.581
• Storage Temp.: -15°C
• CAS DataBase Reference: Z-ALA-ALA-LEU-PNA(CAS DataBase Reference)
• NIST Chemistry Reference: Z-ALA-ALA-LEU-PNA(61043-33-2)
• EPA Substance Registry System: Z-ALA-ALA-LEU-PNA(61043-33-2)

Safety Data

• Hazardous Substances Data: 61043-33-2(Hazardous Substances Data)

Usage

General Description

Z-ALA-ALA-LEU-PNA, also known as Z-AALPNA, is a chemical compound consisting of the amino acids alanine, leucine, and aminohexanoic acid (PNA). It is commonly used as a substrate for studying enzymatic activities, particularly for proteases that cleave after leucine residues. Z-AALPNA is a synthetic peptide that has a fluorescent reporter group attached to it, allowing for easy detection and measurement of protease activity. Its specificity for leucine residues makes it a valuable tool in research for identifying and characterizing protease enzymes. Additionally, Z-AALPNA has applications in drug discovery and development, particularly in the screening and profiling of protease inhibitors and modulators.

InChI:InChI=1/C26H33N5O7/c1-16(2)14-22(25(34)29-20-10-12-21(13-11-20)31(36)37)30-24(33)17(3)27-23(32)18(4)28-26(35)38-15-19-8-6-5-7-9-19/h5-13,16-18,22H,14-15H2,1-4H3,(H,27,32)(H,28,35)(H,29,34)(H,30,33)/t17-,18-,22-/m0/s1

Upstream product

• 2483-51-4 N-[(benzyloxy)carbonyl]-L-alanyl-L-alanine methyl ester 
• 4178-93-2 (S)-2-amino-4-methyl-N-(4-nitrophenyl)valeramide 
• 16012-70-7 benzyloxycarbonyl-L-alanyl-L-alanine 

Downstream Products

• 54865-22-4 N-benzyloxycarbonyl-alanyl-alanyl-leucine 
• 100-01-6 4-nitro-aniline 

Relevant articles and documents

Peptide synthesis in organic media with the use of subtilisin 72 immobilized on a poly(vinyl alcohol) cryogel

Subtilisin 72 serine protease (EC 3.4.21.14) immobilized on a poly(vinyl alcohol) cryogel was used as a catalyst in the syntheses of N-protected peptide p-nitroanilides of the general formulas Z(or Boc)-Xaa-Phe-pNA (Xaa = Leu or Ala), Z-Ala-Xaa-Yaa-pNA (X

SDS-subtilisin complex efficiently catalyzes synthesis of peptides in ethanol and 2-propanol

An enzymatic synthesis of tripeptide Z-Ala-Ala-Leu-pNA, tetrapeptides Z-Ala-Ala-P1-P1'-Xaa, where P1 = Leu, Trp, Met, Ala, Ile, Phe; P1'= Phe, Ala, Leu; Xaa = pNA, NH2, pentapeptides Z-Ala-Ala-Leu-Ala

Biocatalytic properties of thermolysin immobilized on polyvinyl alcohol cryogel

Preparations with different contents of thermolysin were obtained by the immobilization of the enzyme on granulated polyvinyl alcohol cryogel. Their activity and stability in an aqueous medium and in mixtures of polar organic solvents of different composition were investigated. The catalytic properties of the preparations in reactions of peptide bond formation were studied, and the optimal amount of the biocatalyst, the concentrations of initial reagents, and the ratios of organic solvents and water necessary for effective enzymatic peptide synthesis catalyzed by immobilized thermolysin were determined. A series of peptides of the general formula Z-Ala-Ala-Xaa-pNA, where Xaa = Leu, Ile, Phe, Val, or Ala, were synthesized, and the immobilized enzyme was shown to retain substrate specificity in an organic medium.