62253-63-8

Basic information

• Product Name: Epidermal Growth Factor
• Synonyms: RECOMBINANT MOUSE EGF;RECOMBINANT HUMAN EGF;REC EPIDERMAL GROWTH FACTOR (HUMAN);REC EGF (HUMAN);RHEGF;MURINE URG;MURINE UROGASTRONE;MOUSE EGF
• CAS NO: 62253-63-8
• Molecular Formula: C270H401N73O83S7
• Molecular Weight: 6221.96724
• EINECS: 263-468-7
• Product Categories: Cytokine
• Mol File: 62253-63-8.mol
• Article Data: 0

Chemical Properties

• Appearance: /powder
• Storage Temp.: 2-8°C
• CAS DataBase Reference: Epidermal Growth Factor(CAS DataBase Reference)
• NIST Chemistry Reference: Epidermal Growth Factor(62253-63-8)
• EPA Substance Registry System: Epidermal Growth Factor(62253-63-8)

Safety Data

• WGK Germany: 3
• RTECS: KB4518000
• F: 10-21
• Hazardous Substances Data: 62253-63-8(Hazardous Substances Data)

Usage

Description

An ophthalmic formulation of epidermal growth factor (urogastrone) has been launched for stimulation of healing of the corneal epithelium following various corneal diseases.

Chemical Properties

Endotoxin < 0.1 ng per μg (1EU/ug) of EGF

Originator

Inpharzam (Italy)

Uses

Different sources of media describe the Uses of 62253-63-8 differently. You can refer to the following data:
1. Epidermal Growth Factor, human, animal component free has been used: as a supplement in in LHC-8 medium to culture liver cell lines in the fetal bovine serum (FBS)-Dulbecco′s modified essential medium (DMEM) /F12 medium for primary culture of human glioma cells as an additive in the conditional medium of normal fibroblasts (NFs) to study its effect on the migration and invasion of endometrial cancer (EC) cellsas a component in tumorsphere medium
2. EGF (epidermal growth factor) human has been used in the culture medium for the maintenance of MCF10A cells (human mammary epithelial cells).
3. This product is used as a mitogen, triggering the cell to commence mitosis, in a variety of cell lines. In tissue cultures, EGF acts to reduce or eliminate the requirement for serum and can be used in conjunction with other media additives and hormones.

Brand name

Gentel

General Description

Epidermal Growth Factor (EGF) is a small mitogenic polypeptide (～6 kDa), which is present in many mammalian species and is distributed throughout a wide number of tissues and body fluids.? Human EGF is identical to β-urogastrone, a polypeptide which was recognized and isolated on the basis of its ability to inhibit gastric acid secretion.? EGF is a member of a growth factor family, which is characterized by the presence of 6 conserved cysteine motifs that form three disulfide bonds. The location of 3 intrachain disulfide bonds in recombinant human EGF is identical to that of mouse EGF.? EGF is homologous to a sequence contained in a 19 kDa protein of vaccinia virus,? which appears to utilize the EGF receptor to gain entry into cell? EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa cells, and SV40-3T3 cells.?

Biochem/physiol Actions

Epidermal growth factor (EGF) helps to induce cell growth, proliferation and differentiation. It participates in the repairing of renal tissues in the kidney. It promotes the reabsorption of magnesium with the help of the transient receptor potential cation channel 6 (TRMP6). EGF is known to participate in the pathophysiology of drug-induced renal magnesium loss.