64145-26-2Relevant academic research and scientific papers
Synthesis of deoxyadenosine-5′-(35S)thiomonophosphate [dAMP(35S)] of high specific activity - A key intermediate for the synthesis of Sp-deoxyadenosine-5′-(α-35S)thiotriphosphate [Sp-dATP (α-35S)]
Mathew,Ravi,Sontakke,Chander
, p. 19 - 28 (2002)
Unprotected deoxyadenosine 1 was treated with an excess of phosphorus acid and stoichiometric proportions of N, N′-di-p- tolylcarbodiimide in anhydrous pyridine to give deoxyadenosine-5′-monophosphite 2. The latter wax activated with trimethylsilyl chloride followed by sulphurisation with elemental 35S (specific activity > 1000 Ci/mmol) in toluene solution to give deoxyadenosine5′-(35S)-thiomonophosphate [dAMP(35S)] 3. Enzymatic conversion of deoxyadenosine-5′-(35S)-thiomonophosphate to Sp-deoxyadenosine-5′-(α-35S)-thiotriphosphate [Sp-dATP (α-35S)] 5 was carried out following a standard reaction protocol. Copyright
An efficient reagent for the phosphorylation of deoxyribonucleosides, DNA oligonucleotides, and their thermolytic analogues
Ausin, Cristina,Grajkowski, Andrzej,Cieslak, Jacek,Beaucage, Serge L.
, p. 4201 - 4204 (2007/10/03)
(Chemical Equation Presented) The phosphoramidite 11 was prepared in three steps from methyl 2-mercaptoacetate and demonstrated efficiency in the synthesis of conventional 5′-/3′-phosphate/thiophosphate monoester derivatives of 2′-deoxyribonucleosides and DNA oligonucleotides. Moreover, the use of 11 has enabled the preparation of the dinucleoside phosphorothioate analogue 26 in high yields (>95%) with minimal cleavage (2%) of the thermolytic thiophosphate protecting group.
