642475-14-7Relevant academic research and scientific papers
In vitro lipofection with novel series of symmetric 1,3-dialkoylamidopropane-based cationic surfactants containing single primary and tertiary amine polar head groups
Sheikh, Mohammad,Feig, Jennifer,Gee, Becky,Li, Song,Savva, Michalakis
, p. 49 - 61 (2003)
A novel series of symmetric double-chained primary and tertiary 1,3-dialkoylamido monovalent cationic lipids were synthesized and evaluated for their transfection activities. In the absence of the helper lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine), only the primary and tertiary dioleoyl derivatives 1,3lmp5 and 1,3lmt5, respectively elicited transfection activity. This is a striking difference between symmetrical 1,2-diacyl glycerol-based monovalent cationic lipids that always found both dioleoyl and dimyristoyl analogues being efficient transfection reagents. In the presence of helper lipid, all cationic derivatives induced marker gene expression, except the dilauroyl analogues 1,3lmp1 and 1,3lmt1 that elicited no transfection activity. Combining electrophoretic mobility data of the lipoplexes at different charge ratios with transfection activity suggested two requirements for high transfection activity with monovalent double-chained cationic lipids, that is, binding/association of the lipid to the plasmid DNA and membrane fusion properties of the lipid layers surrounding the DNA.
NUCLEIC ACID DERIVATIVE HAVING IMMUNOSTIMULATORY ACTIVITY
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Paragraph 0325; 0353; 0354, (2018/11/02)
The purpose of the present invention is to provide double-stranded oligonucleotides comprising the CpG oligonucleotide mentioned below, as a nucleic acid derivative having an immunostimulatory activity. An adjuvant comprising a double-stranded oligonucleo
Single-component pH-sensitive liposomes of reduced solid-to-liquid phase transition temperatures
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Page/Page column 3, (2008/06/13)
The current invention relates to the synthesis of novel cationic lipids and their use as delivery vectors for nucleic acids, peptides and other synthetic drugs, in vitro and in vivo. The cationic lipids described herein form stable lamellar structures (liposomes) at physiological pH but destabilize to micelles at acidic and alkaline pH. These structures are characterized of high elasticity, increased fluidity and high transfection activity relative to the corresponding 1,2-dialkyl cationic derivatives and other phospholipids analogues.
