64396-27-6Relevant articles and documents
Biochemical characterization of Helicobacter pylori α1–3-fucosyltransferase and its application in the synthesis of fucosylated human milk oligosaccharides
Bai, Jing,Wu, Zhigang,Sugiarto, Go,Gadi, Madhusudhan Reddy,Yu, Hai,Li, Yanhong,Xiao, Cong,Ngo, Alice,Zhao, Baohua,Chen, Xi,Guan, Wanyi
, p. 1 - 6 (2019/05/28)
Fucosylated human milk oligosaccharides (HMOs) have important biological functions. Enzymatic synthesis of such compounds requires robust fucosyltransferases. A C-terminal 66-amino acid truncated version of Helicobacter pylori α1–3-fucosyltransferase (Hp3FT) is a good candidate. Hp3FT was biochemically characterized to identify optimal conditions for enzymatic synthesis of fucosides. While N-acetyllactosamine (LacNAc) and lactose were both suitable acceptors, the former is preferred. At a low guanosine 5′-diphospho-β-L-fucose (GDP-Fuc) to acceptor ratio, Hp3FT selectively fucosylated LacNAc. Based on these enzymatic characteristics, diverse fucosylated HMOs, including 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP) III, lacto-N-neofucopentaose (LNnFP) V, lacto-N-neodifucohexaose (LNnDFH) II, difuco- and trifuco-para-lacto-N-neohexaose (DF-paraLNnH and TF-para-LNnH), were synthesized enzymatically by varying the ratio of the donor and acceptor as well as controlling the order of multiple glycosyltransferase-catalyzed reactions.
Glycosyl Imidates, 49 - Synthesis of the Octasaccharide Moiety of the Dimeric Lex Antigen
Bommer, Rene,Kinzy, Willy,Schmidt, Richard R.
, p. 425 - 434 (2007/10/02)
A versatile synthesis of the basic Galβ(1->4)3)>-GlcNAc trisaccharide building block of the Lex antigen family based on two important findings could be developed.Firstly, selective 3-O-allylation of the readily available azidolactose d