64431-96-5 Usage
Uses
Used in Diagnostic Assay Manufacturing Industry:
1,3-Bis[tris(hydroxymethyl)methylamino]propane is used as a buffer for its wide pH range of 6.3 9.5, which is attributed to its two pKa values, 6.8 (pKa1) and 9.0 (pKa2), being close to each other. This wide buffering range, especially down to pH 6 7, makes it an ideal choice for enhancing the stability or activity of restriction enzymes, as compared to Tris buffer, which is a poor buffer below pH 7.5 and has a comparatively large change in pKa with temperature.
Used in Investigating the Influence of Anions on Autoxidation of Oxymyoglobin:
1,3-Bis[tris(hydroxymethyl)methylamino]propane is used as a buffer to study the impact of anions with varying nucleophilic properties on the autoxidation of oxymyoglobin (MbO2).
Used in Purification of Glucose-Binding Protein:
In the field of protein purification, 1,3-Bis[tris(hydroxymethyl)methylamino]propane serves as a buffer during the purification process of glucose-binding protein from the membranes of Sulfolobus solfataricus.
Used in GeO2 Mineralisation:
1,3-Bis[tris(hydroxymethyl)methylamino]propane is utilized as a buffer during the mineralisation process of GeO2, playing a crucial role in maintaining the pH stability required for the reaction to proceed efficiently.
Precautions
This trihydroxyamine analog, also known as bis-tris propane, presents as a white to almost white crystalline powder. Store in a cool and dark place. 1,3-Bis[tris(hydroxymethyl)methylamino]propane is known to be incompatible with oxidizing agents and should not be handled in their vicinity.
Check Digit Verification of cas no
The CAS Registry Mumber 64431-96-5 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 6,4,4,3 and 1 respectively; the second part has 2 digits, 9 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 64431-96:
(7*6)+(6*4)+(5*4)+(4*3)+(3*1)+(2*9)+(1*6)=125
125 % 10 = 5
So 64431-96-5 is a valid CAS Registry Number.
InChI:InChI=1/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2
64431-96-5Relevant academic research and scientific papers
Mechanistic information from pressure acceleration of hydride formation via proton binding to a cobalt(I) macrocycle
Fujita, Etsuko,Wishart, James F.,Van Eldik, Rudi
, p. 1579 - 1583 (2008/10/08)
The effect of pressure on proton binding to the racemic isomer of the cobalt(I) macrocycle, CoL+ (L = 5,7,7,12.-14, 14-hexamethyl-1,4,8,11-tetraazacyclotetradeca-4,11-diene), has been studied for a series of proton donors using pulse radiolysis techniques. The second-order rate constants for the reaction of CoL+ with proton donors decrease with increasing pKa of the donor acid, consistent with a reaction occurring via proton transfer. Whereas the corresponding volumes of activation (ΔV?) are rather small and negative for all acids (proton donors) with pKa values below 8.5, significantly larger negative activation volumes are found for weaker acids (pKa> 9.5) containing OH groups as proton donors. In the latter case, the observed ΔV? for these protonation reactions show a correlation with the reaction volumes (ΔV°ion) for the ionization of the weak acids with a slope of 0.44, indicating that bond dissociation of the weak acid molecule bound to the metal center proceeds approximately halfway at the transition state along the reaction coordinate in terms of volume changes.
Therapeutically active complexes of tetracyclines
-
, (2008/06/13)
Tri-tetracyclines are complexes of tetracyclines with an amino compound containing per molecule at least a tris (hydroxyalkyl) group and at least a primary or secondary amino group in a molar excess in respect to the tetracycline compound. The complexes have high water solubility and are particularly useful for parenteral administration.
