64431-96-5 Usage
Description
BIS-TRIS Propane is Good′s buffer. It acts as ligand and forms series of dinuclear hydroxo complexes with Lanthanides(III) in the pH range 7-9. It is useful for diagnostic assay manufacturing industry.
Chemical Properties
White powder
Uses
Different sources of media describe the Uses of 64431-96-5 differently. You can refer to the following data:
1. BIS-TRIS Propane may be used in the following studies:As buffer to investigate the influence of anions with varying nucleophilic properties on the autoxidation of oxymyoglobin (MbO2).Purification of glucose-binding protein from membranes of Sulfolobus solfataricus.As buffer during GeO2 mineralisation.
2. BIS-Tris Propane is a buffer that is useful in the pH 6.3 - 9.5 range. This wide buffering range is due to its two pKa values, 6.8 (pKa1) and 9.0 (pKa2), being so close. Because of the wide buffering range, particularly down to pH 6 - 7, this buffer has been used to enhance the stability or activity of restriction enzymes, compared to Tris buffer (which is a poor buffer below pH 7.5 and has a comparatively large change in pKa with temperature).
Application
BIS-TRIS Propane may be used in the following studies:As buffer to investigate the influence of anions with varying nucleophilic properties on the autoxidation of oxymyoglobin (MbO2).Purification of glucose-binding protein from membranes of Sulfolobus solfataricus.As buffer during GeO2 mineralisation.
Precautions
This trihydroxyamine analog, also known as bis-tris propane, presents as a white to almost white crystalline powder. Store in a cool and dark place. 1,3-Bis[tris(hydroxymethyl)methylamino]propane is known to be incompatible with oxidizing agents and should not be handled in their vicinity.
Check Digit Verification of cas no
The CAS Registry Mumber 64431-96-5 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 6,4,4,3 and 1 respectively; the second part has 2 digits, 9 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 64431-96:
(7*6)+(6*4)+(5*4)+(4*3)+(3*1)+(2*9)+(1*6)=125
125 % 10 = 5
So 64431-96-5 is a valid CAS Registry Number.
InChI:InChI=1/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2
64431-96-5Relevant articles and documents
Mechanistic information from pressure acceleration of hydride formation via proton binding to a cobalt(I) macrocycle
Fujita, Etsuko,Wishart, James F.,Van Eldik, Rudi
, p. 1579 - 1583 (2008/10/08)
The effect of pressure on proton binding to the racemic isomer of the cobalt(I) macrocycle, CoL+ (L = 5,7,7,12.-14, 14-hexamethyl-1,4,8,11-tetraazacyclotetradeca-4,11-diene), has been studied for a series of proton donors using pulse radiolysis techniques. The second-order rate constants for the reaction of CoL+ with proton donors decrease with increasing pKa of the donor acid, consistent with a reaction occurring via proton transfer. Whereas the corresponding volumes of activation (ΔV?) are rather small and negative for all acids (proton donors) with pKa values below 8.5, significantly larger negative activation volumes are found for weaker acids (pKa> 9.5) containing OH groups as proton donors. In the latter case, the observed ΔV? for these protonation reactions show a correlation with the reaction volumes (ΔV°ion) for the ionization of the weak acids with a slope of 0.44, indicating that bond dissociation of the weak acid molecule bound to the metal center proceeds approximately halfway at the transition state along the reaction coordinate in terms of volume changes.