64480-74-6Relevant academic research and scientific papers
Identification of urinary metabolites of orally administered N,N-dimethyl-p-toluidine in male F344 rats
Kim, Nam-Cheol,Ghanbari, Katayoon,Kracko, Dean A.,Weber, Waylon M.,McDonald, Jacob D.,Dix, Kelly J.
, p. 781 - 788 (2008/02/02)
The metabolism of orally administered N,N-dimethyl-p-toluidine (DMPT) in male F344 rats was investigated. The rat urinary metabolite profile was determined by analytical reverse-phase high performance liquid chromatography (HPLC). Four radiolabeled peaks were observed, isolated, and purified by solid-phase extraction (SPE) and preparative HPLC methods. The 4 peaks were identified as p-(N-acetylhydroxyamino)hippuric acid (M1), DMPT N-oxide (M2), N-methyl-p-toluidine (M3), and parent DMPT. Metabolites M1 and M2 were identified by spectrometric and spectroscopic methods, including mass fragmentation pattern identification from both liquid chromatography/mass spectrometry and gas chromatography/mass spectrometry, and from chemical analysis of nuclear magnetic resonance spectra. Structural confirmation of metabolite M2 was accomplished by comparison with a synthetic standard. Peaks M3 and the peak suspected to be DMPT were identified by comparison of their HPLC retention times and mass fragmentation patterns with authentic standards of N-methyl-p-toluidine and DMPT, respectively. DMPT metabolism is similar to that reported for N,N-dimethylaniline. Copyright Taylor & Francis Group, LLC.
Biomimetic catalysis of intermodular aminoacyl transfer
Wilcoxen, Keith M.,Leman, Luke J.,Weinberger, Dana A.,Huang, Zheng-Zheng,Ghadiri, M. Reza
, p. 748 - 749 (2007/10/03)
Intermodular aminoacyl transfer is the fundamental bond-forming reaction in the biosynthesis of polypeptides by ribosomes and nonribosomal peptide synthetases (NRPS). Here we report the design and functional characterizations of short synthetic α-helical
