64486-64-2

Usage

InChI:InChI=1/C12H26N2O/c1-11(2)8-10(14(5,6)7)9-12(3,4)13(11)15/h10H,8-9H2,1-7H3/q+1

Upstream product

• 74-87-3 methylene chloride 
• 32327-90-5 4-dimethylamino-2,2,6,6-tetramethyl piperidine 
• 1028569-82-5 1-hydroxy-2,2,6,6-tetramethylpiperidin-4-yltrimethylammonium chloride 

Relevant academic research and scientific papers

A SOLUTION OF TEMPO-DERIVATIVES FOR USE AS ELECTROLYTE IN REDOX-FLOW CELLS

The present invention relates to a solution comprising water and different 2,2,6,6-tetramethyl-piperidinyl-oxyl (TEMPO)-derivatives, a process for the production of this solution, a process for making a redox-flow cell comprising the solution as electrolyte, the redox-flow cell comprising the solution as an electrolyte in one chamber of the cell and the use of the redox-flow cell for storing electrical energy.

EPR detection of cellular and mitochondrial superoxide using cyclic hydroxylamines

Superoxide (O2) has been implicated in the pathogenesis of many human diseases, but detection of the O2 radicals in biological systems is limited due to inefficiency of O2 spin trapping and lack of site-specific information. This work studied production of extracellular, intracellular and mitochondrial O2 in neutrophils, cultured endothelial cells and isolated mitochondria using a new set of cationic, anionic and neutral hydroxylamine spin probes with various lipophilicity and cell permeability. Cyclic hydroxylamines rapidly react with O2, producing stable nitroxides and allowing site-specific O2 detection in intracellular, extracellular and mitochondrial compartments. Negatively charged 1-hydroxy-4-phosphono-oxy-2,2,6,6-tetramethylpiperidine (PP-H) and positively charged 1-hydroxy-2,2,6,6-tetramethylpiperidin-4-yl-trimethylammonium (CAT1-H) detected only extramitochondrial O2. Inhibition of EPR signal by SOD2 over-expression showed that mitochondria targeted mitoTEMPO-H detected intramitochondrial O2 both in isolated mitochondria and intact cells. Both 1-hydroxy-3-carboxy-2,2,5,5-tetramethylpyrrolidine (CP-H) and 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine (CM-H) detected an increase in cytoplasm O2 stimulated by PMA, but only CM-H and mitoTEMPO-H showed an increase in rotenone-induced mitochondrial O2. These data show that a new set of hydroxylamine spin probes provide unique information about site-specific production of the O2 radical in extracellular or intracellular compartments, cytoplasm or mitochondria.