645-15-8

Usage

Uses

1. Used in Enzyme Activity Determination:
Bis(4-nitrophenyl) phosphate is used as a substrate for determining the enzyme activity of root phosphodiesterases in wetland plants. This application aids in understanding the role and efficiency of these enzymes in the ecological processes of these plants.
2. Used in Mechanism of Cleavage Studies:
BNPP is employed in studying the mechanism of its own cleavage using oxamido-bridged dinuclear copper(II) complexes as catalysts. This research contributes to the broader understanding of chemical reactions and the potential applications of BNPP in various fields.
3. Used as an Enzyme Inhibitor:
Bis(4-nitrophenyl) phosphate serves as an inhibitor of certain enzymes, specifically for phosphodiesterase substrates. This application is crucial in the development of pharmaceuticals and therapies targeting specific enzymatic pathways.

InChI:InChI=1/C12H9N2O8P/c15-13(16)9-1-5-11(6-2-9)21-23(19,20)22-12-7-3-10(4-8-12)14(17)18/h1-8H,(H,19,20)/p-1

Upstream product

• 100-02-7 4-nitro-phenol 
• 838-85-7 diphenyl hydrogen phosphate 
• 3871-20-3 tris(p-nitrophenyl)phosphate 
• 2015-56-7 diphenylphosphoroamidate 
• 7697-37-2 nitric acid 

Downstream Products

• 64108-81-2 phosphoric acid benzyl ester-bis-(4-nitro-phenyl ester) 
• 102048-77-1 O^2',O^3'-isopropylidene-[5']guanylic acid bis-(4-nitro-phenyl ester) 
• 111269-96-6 O¹,O²-isopropylidene-O³,O⁵-(4-nitro-phenoxyphosphoryl)-α-D-xylofuranose 
• 72779-21-6 phosphoric acid 4-nitro-phenyl ester prop-2-ynyl ester 
• 71465-03-7 Octadecanoic acid 2-[bis-(4-nitro-phenoxy)-phosphoryloxy]-1-octadecanoyloxymethyl-ethyl ester 
• 72011-66-6 phosphoric acid 2-methoxy-ethyl ester 4-nitro-phenyl ester 
• 6546-97-0 bis(4-nitrophenyl) phosphorochloridate 
• 100-02-7 4-nitro-phenol 
• 92220-88-7 thymidine 5'- 
• 330-13-2 mono-p-nitrophenyl phosphate 
• 799-87-1 bis(p-nitrophenyl) methyl phosphate 
• 36199-67-4 4-nitrophenyl phosphate 
• 407578-85-2 bis(4-aminophenyl)phosphoric acid 
• 15930-83-3 methyl (4-nitrophenyl)phosphate 

Relevant academic research and scientific papers

Pentacoordinate Nickel(II) Complexes Double Bridged by Phosphate Ester of Phosphinate Ligands: Spectroscopic, Structural, Kinetic and Magnetic Studies

The bis(phosphatediester)-bridged complexes [{Ni([12]aneN 3)(μ-O2P(OR)2)}2](PF 6)2 {[12]aneN3= Me3[12]aneN 3, 2,4,4-trimethyl-1,5,9-triazacyclododec-1-ene; R=Me (1), Bu (2), Ph (3), Ph-4-NO2 (4); [12]aneN3= Me 4[12]aneN3, 2,4,4,9-tetramethyl-1,5,9-triazacyclododec-1-ene; R=Me (5), Bu (6), Ph (7), Ph-4-NO2 (8)} were prepared by hydrolysis of the phosphate triester with the hydroxo complex [{Ni([12]aneN3)(μ-OH)} 2](PF6)2 or by acid-base reaction of the dialkyl or diaryl phosphoric acid and the above hydroxo complex. The acid-base reaction was also used to synthesise the phosphinate-bridged complexes [{Ni([12]aneN3)(μ-O2PR2)} 2](PF6)2 {[12]aneN3=Me 3[12]aneN3, R=Me (9), Ph (10); [12]aneN 3=Me4[12]aneN3, R=Me (11), Ph (12)}. The molecular structures of complexes 2, 3 and 12 were established by single crystal X-ray diffraction studies. The eight-membered rings defined by the nickel atoms and the bridging ligands show distorted twist-boat, chair and boat-boat conformations in 2, 3 and 12, respectively. The experimental susceptibility data for compounds 2, 3 and 12 were fitted by least-squares methods to the analytical expression given by Ginsberg. The best fit was obtained with values of J= -0.11 cm-1, D=-9.5 cm-1 and g=2.20 for 2; J=-0.97 cm-1, D=-9.3 cm-1 and g=2.21 for 3; and J=-0.14 cm-1, D=-11.9 cm-1 and g=2.195 for 12. The magnetic-exchange pathways must involve the phosphate/phosphinate bridges, because these favour antiferromagnetic interactions. The observation of a higher exchange parameter for compound 3 is a consequence of a favourable disposition of the O-P-O bridges. The kinetics for the hydrolysis of TNP (tris(4-nitrophenyl)phosphate) with the dinuclear nickel(II) hydroxo complex [{Ni(Me3[12]aneN3)(μ-OH)}2]-(PF 6)2 was studied by UV-visible spectroscopy. The proposed mechanism for TNP-promoted hydrolysis can be described as one-substrate/two-product, and can be fitted to a Michaelis-Menten equation.

Hydrolytic function of a hydroxo-ZnIIPbII complex toward tris-p-nitrophenyl phosphate: A functional model of heterobimetallic phosphatases

A ZnIIPbII complex ZnPb(L)(H2O)2(ClO4)2 of a macrocyclic compartmental ligand L2- is converted under alkaline conditions into a hydroxo complex ZnPb(L)(OH)(H2O)ClO4 that hydrolyzes tris-p-nitrophenyl phosphate (TNP) to bis-p-nitrophenyl phosphate (BNP-) forming a BNP-bridged complex [ZnPb(L)(BNP)]ClO4.

Photo-enhanced hydrolysis of bis(4-nitrophenyl) phosphate using Cu(ii) bipyridine-capped plasmonic nanoparticles

We show that the hydrolysis of bis(4-nitrophenyl) phosphate by a Cu(ii) bipyridine complex was enhanced by 1000-fold when covalently attached to 10 nm gold nanoparticles (AuNP) and irradiated with a 120 mW green laser at 532 nm when compared to an unsuppo

Synthesis and structures of soluble magnesium and zinc carboxylates containing intramolecular NH?O hydrogen bonds in nonpolar solvents

Readily soluble magnesium and zinc carboxylates [M(Ln)2(H2O)4] (MLn2) (M = Mg, Zn; L1 = O2C-2-Ar3CCONH-6-n-BuCONHC6H3; L2 = O2C-2-Ar3CCONHC6H4; Ar = 4-t-BuC6H4) containing intramolecular NH?O hydrogen bonds in nonpolar solvents were synthesized and their molecular structures were determined by X-ray analysis. The complexes were crystallized in the trans or cis configuration. The M-O bond distances were dependent on the mode of the hydrogen bonds. 1H NMR spectral measurements revealed a fast trans-cis isomerization of MLn2 in CDCl3, which was converted into a unique fac-[M(Ln)3(H2O)3]- (fac-[MLn3]-) by the addition of equimolar [Ln]-. The theoretical calculations supported the existence of the facial configuration. The coordinated water molecules of MLn2 were detected by 1H NMR spectroscopy and the acidity was estimated in the order of ML12 > ML22. Calcium afforded only the dinuclear complex, [Ca2(L1)2(H2O)5(1,4-dioxane)] (Ca2L14), which showed a low hydrolytic activity.

Regulation of the hydrolytic activity of Mg2+-dependent phosphatase models by intramolecular NH···O hydrogen bonds

Magnesium-dependent phosphatase models containing intramolecular NH···O hydrogen bonds were synthesized and structurally characterized by X-ray analysis. The Mg-O bond distances varied with the mode of the hydrogen bonds. 1H NMR spectra in nonp