6450-77-7 Usage
Uses
1-[5-[[[[5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxymethyl]-3,4-dihydroxy-oxolan-2-yl]pyridine-5-carboxylate is used as a coenzyme in redox reactions for its ability to act as a donor of ADP-ribose moieties and as a precursor of cyclic ADP-ribose.
Used in Enzyme Regulation:
In the pharmaceutical industry, 1-[5-[[[[5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxymethyl]-3,4-dihydroxy-oxolan-2-yl]pyridine-5-carboxylate is used as a substrate for bacterial DNA ligases and sirtuins, which are enzymes that play a crucial role in various cellular processes, including DNA repair and protein deacetylation.
Used in Research Applications:
In the field of scientific research, 1-[5-[[[[5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxymethyl]-3,4-dihydroxy-oxolan-2-yl]pyridine-5-carboxylate serves as an important tool for studying the mechanisms of redox reactions, ADP-ribosylation, and the role of NAD+ in cellular processes.
Used in Drug Development:
1-[5-[[[[5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxymethyl]-3,4-dihydroxy-oxolan-2-yl]pyridine-5-carboxylate may also be utilized in the development of novel therapeutic agents targeting enzymes and pathways that involve NAD+, potentially leading to new treatments for various diseases and conditions.
Check Digit Verification of cas no
The CAS Registry Mumber 6450-77-7 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 6,4,5 and 0 respectively; the second part has 2 digits, 7 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 6450-77:
(6*6)+(5*4)+(4*5)+(3*0)+(2*7)+(1*7)=97
97 % 10 = 7
So 6450-77-7 is a valid CAS Registry Number.
InChI:InChI=1/C21H26N6O15P2/c22-17-12-18(24-7-23-17)27(8-25-12)20-16(31)14(29)11(41-20)6-39-44(36,37)42-43(34,35)38-5-10-13(28)15(30)19(40-10)26-3-1-2-9(4-26)21(32)33/h1-4,7-8,10-11,13-16,19-20,28-31H,5-6H2,(H4-,22,23,24,32,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,19-,20-/m1/s1
6450-77-7Relevant academic research and scientific papers
A universal, fully automated high throughput screening assay for pyrophosphate and phosphate release from enzymatic reactions
Pegan, Scott D.,Tian, Yang,Sershon, Valerie,Mesecar, Andrew D.
experimental part, p. 27 - 38 (2010/08/07)
The malachite green assay is often used for measuring the presence of inorganic mono-phosphate concentrations. Some studies have adapted this assay for use in monitoring enzymatic reactions and have suggested its potential use in high throughput screening (HTS). With the increasing availability of laboratory automation, some studies are starting to explore the possibility of conducting limited, semi-automated versions of the assay. Here we report the optimization and complete adaptation of the malachite green assay to a fully automated, HTS platform that can be performed unattended with standard, commercially available, automated liquid-handling systems. The assay is universal for the majority of enzymes that release phosphate or pyrophosphate. Moreover, the assay is fully scalable from smaller drug screening efforts (～20,000 wells per day) to ultra-high throughput environments (～200,000 wells per day). The assay uses cost-effective, commercially available reagents, and can be used to perform automated IC50 value and kinetic parameter determination. Finally, we demonstrate the utility of the assay via the initial, primary screening of 100,080 compounds against two target enzymes from Bacillus anthracis, O-succinylbenzoyl-CoA synthetase and nicotinate mononucleotide adenylyltransferase.
