Z-Pro-Gly-ONp, also known as N-benzyloxycarbonyl-L-prolyl-glycine p-nitrophenyl ester, is a synthetic tripeptide derivative commonly used in biochemical research and assays. It consists of three amino acids: L-proline, glycine, and a p-nitrophenyl ester group. The "Z" in the name stands for the benzyloxycarbonyl (Z) protecting group, which is often used to protect the amino group of the peptide during synthesis. Z-Pro-Gly-ONp is particularly useful for studying protease enzymes, as it serves as a substrate that can be cleaved by these enzymes, releasing the p-nitrophenol moiety upon hydrolysis. The release of p-nitrophenol can be easily quantified by measuring the absorbance at 410 nm, making Z-Pro-Gly-ONp a valuable tool for assessing protease activity and specificity in various biological and medicinal applications.
The CAS Registry Mumber 6464-84-2 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 6,4,6 and 4 respectively; the second part has 2 digits, 8 and 4 respectively. Calculate Digit Verification of CAS Registry Number 6464-84: (6*6)+(5*4)+(4*6)+(3*4)+(2*8)+(1*4)=112 112 % 10 = 2 So 6464-84-2 is a valid CAS Registry Number.