65062-64-8Relevant academic research and scientific papers
PHOSPHOCYTIDINES AS VERSATILE 3' PROTECTING GROUPS IN TRIESTER SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES
Gough, G.R.,Brunden, M.J.,Gilham, P.T.
, p. 5317 - 5320 (1983)
Any oligodeoxyribonucleotide triester block with a 3' aryl phosphate end can be protected by condensing its phosphodiester terminal with the 2'-hydroxyl group of N4,O3',05'-tribenzoylcytidine.As the final step in deprotection of the modified block, mild treatment with Pb++ ion cleaves the new 3'-2' linkage in such a way that the original 3' terminal phosphate group of the oligomer is transferred to the ribonucleoside.This procedure eliminates the need for specialized terminator blocks in triester synthesis.
