Tetrahedron Letters p. 5317 - 5320 (1983)
Update date:2022-08-05
Topics:
Gough, G.R.
Brunden, M.J.
Gilham, P.T.
Any oligodeoxyribonucleotide triester block with a 3' aryl phosphate end can be protected by condensing its phosphodiester terminal with the 2'-hydroxyl group of N4,O3',05'-tribenzoylcytidine.As the final step in deprotection of the modified block, mild treatment with Pb++ ion cleaves the new 3'-2' linkage in such a way that the original 3' terminal phosphate group of the oligomer is transferred to the ribonucleoside.This procedure eliminates the need for specialized terminator blocks in triester synthesis.
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