PHOSPHOCYTIDINES AS VERSATILE 3' PROTECTING GROUPS IN TRIESTER SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES

Article abstract of DOI:10.1016/S0040-4039(00)87857-0

Any oligodeoxyribonucleotide triester block with a 3' aryl phosphate end can be protected by condensing its phosphodiester terminal with the 2'-hydroxyl group of N⁴,O^3',0^5'-tribenzoylcytidine.As the final step in deprotection of the modified block, mild treatment with Pb⁺⁺ ion cleaves the new 3'-2' linkage in such a way that the original 3' terminal phosphate group of the oligomer is transferred to the ribonucleoside.This procedure eliminates the need for specialized terminator blocks in triester synthesis.