65118-58-3Relevant academic research and scientific papers
Competition between model protocells driven by an encapsulated catalyst
Adamala, Katarzyna,Szostak, Jack W.
, p. 495 - 501 (2013)
The advent of Darwinian evolution required the emergence of molecular mechanisms for the heritable variation of fitness. One model for such a system involves competing protocell populations, each consisting of a replicating genetic polymer within a replic
Highly concentrated water-in-oil emulsions as novel reaction media for protease-catalysed kinetically controlled peptide synthesis
Clapes,Espelt,Navarro,Solans
, p. 1394 - 1399 (2007/10/03)
High-internal-phase-ratio-emulsions (HIPREs) or gel emulsions, formulated with a large amount of water (80.0-99.5% w/w), were investigated as reaction media for α-chymotrypsin-catalysed peptide synthesis under kinetic control using Ac-3-Phe-OEt and H-3-Le
Cross-linked crystals of subtilisin: Versatile catalyst for organic synthesis
Wang, Yi-Fong,Yakovlevsky, Kirill,Zhang, Bailing,Margolin, Alexey L.
, p. 3488 - 3495 (2007/10/03)
Cross-linked enzyme crystals (CLECs) of subtilisin exhibit excellent activity in aqueous and various organic solvents. This catalyst is more stable than the native enzyme in both aqueous and mixed aqueous/organic solutions. Subtilisin-CLEC was shown to be a versatile catalyst. It was used for the syntheses of peptides and peptidomimetics, mild hydrolysis of amino acid and peptide amides, enantio- and regioselective reactions, and transesterifications.
Peptide Synthesis Mediated by Immobilized and Viable Baker's Yeast in Reverse Micelles: Synthesis of Leucine Enkephalin Analogues
Fadnavis, N. W.,Deshpande, A.,Chauhan, S.,Bhalerao, U. T.
, p. 1548 - 1550 (2007/10/02)
Cells of baker's yeast (Saccharomyces cerevisiae NCIM 3305) immobilized in calcium alginate beads are found to be viable in reverse micelles of bis(2-ethylhexyl)sulphosuccinate sodium salt 1 in iso-octane for days and were used for the first time for pept
APPLICATIONS OF ENZYME-CONTAINING REVERSE MICELLES
Leser, Martin E.,Wei, Genshuan,Luethi, Peter,Haering, Gabriel,Hochkoeppler, Alessandro,et al.
, p. 1113 - 1118 (2007/10/02)
This paper is a research report on aspects which are more related to biotechnological application of reverse micelles and water in oil microemulsions.In particular we will consider: (1) the application of enzymes in reverse micelles as catalysts of water insoluble compounds.In this case reverse micelles can be viewed as microreactors, whose dimenisons can be easily changed and with a milieu whose physical properties can be continuously modulated and possibly tailored to the characteristics of the reaction taking place in the water pool; (2) the solubilization of larger particles in water in oil microemulsion, like mitochondria and bacteria, and their possible biotechnological application; (3) the use of reverse micelles to extract and separate proteins.A good specificity of the separation process can be achieved, based on electrostatic effects (pH, pI), even for proteins having quite similar size; (4) the transformation of reverse micellar solutions into "organogels".These novel materials are characterized by a very high viscosity and a significant molecular mobility of the main components (organic solvent, surfactant).Due to the presence of water droplets (or water channels) in the gel, a compartmentalization of hydrophilic biomolecules (also enzymes and/or bacteria) is possible.This is equivalent to the preparation of bioreactors containing immobilized cells or enzymes, which are better compatible with organic solvent.
