65190-39-8Relevant articles and documents
Structure–Activity Relationship Study of a Potent α-Thrombin Binding Aptamer Incorporating Hexitol Nucleotides
De Fenza, Maria,Eremeeva, Elena,Troisi, Romualdo,Yang, Hui,Esposito, Anna,Sica, Filomena,Herdewijn, Piet,D'Alonzo, Daniele,Guaragna, Annalisa
, p. 9589 - 9597 (2020/07/13)
The replacement of one or more nucleotide residues in the potent α-thrombin-binding aptamer NU172 with hexitol-based nucleotides has been devised to study the effect of these substitutions on the physicochemical and functional properties of the anticoagulant agent. The incorporation of single hexitol nucleotides at the T9 and G18 positions of NU172 substantially retained the physicochemical features of the parent oligonucleotide, as a result of the biomimetic properties of the hexitol backbone. Importantly, the NU172-TH9 mutant exhibited a higher binding affinity toward human α-thrombin than the native aptamer and an improved stability even after 24 h in 90 percent human serum, with a significant increase in the estimated half-life. The anticoagulant activity of the modified oligonucleotide was also found to be slightly preferable to NU172. Overall, these results confirm the potential of hexitol nucleotides as biomimetic agents, while laying the foundations for the development of NU172-inspired α-thrombin-binding aptamers.
1,5-anhydrohexitol nucleoside analogues
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, (2008/06/13)
1,5-Anhydrohexitol nucleoside analogues represented by the general formula I: STR1 wherein the hexitol has the D-configuration and the carbon atom on which the base moiety and the X substituent stand both have the (S)-configuration and further wherein: B is a heterocyclic ring selected from the group consisting of pyrimidine and purine bases; and X is hydrogen, azido, F, Cl, Br, I, amino, --NHR2, --N(R2)2, --OR2, --SR2, or CN; wherein R1 and R2 are the same or different and are selected from the group consisting of hydrogen, substituted or unsubstituted C1 -C20 alkyl, substituted or unsubstituted C1 -C20 alkenyl, aroyl, C1 -C20 alkanoyl, and phosphoryl. These nucleoside analogues are useful as antiviral agents against herpes simplex virus, vaccina virus, or varicella zoster virus. The definition of the X group should include --N(R2)2 instead of --N(N2)2.
ANALYSIS BY THE REDUCTIVE-CLEAVAGE METHOD OF LINKAGE POSITIONS IN A POLYSACCHARIDE CONTAINING 4-LINKED D-GLUCOPYRANOSYLURONIC RESIDUES
Vodonik, Sally A.,Gray, Gary R.
, p. 93 - 102 (2007/10/02)
The fate of 4-linked D-glucopyranosyluronic residues under reductive-cleavage conditions was investigated by using the Klebsiella aerogenes type 54 strain A3 capsular polysaccharide.Treatment of the fully methylated polysaccharide with triethylsilane and