66624-69-9Relevant academic research and scientific papers
Structural and functional studies of Aspergillus oryzae cutinase: Enhanced thermostability and hydrolytic activity of synthetic ester and polyester degradation
Zhiqiang, Liu,Gosser, Yuying,Baker, Peter James,Ravee, Yaniv,Ziying, Lu,Alemu, Girum,Huiguang, Li,Butterfoss, Glenn L.,Kong, Xiang-Peng,Gross, Richard,Montclare, Jin Kim
, p. 15711 - 15716 (2009)
Cutinases are responsible for hydrolysis of the protective cutin lipid polyester matrix in plants and thus have been exploited for hydrolysis of small molecule esters and polyesters. Here we explore the reactivity, stability, and structure of Aspergillus oryzae cutinase and compare it to the well-studied enzyme from Fusarium solani. Two critical differences are highlighted in the crystallographic analysis of the A. oryzae structure: (i) an additional disulfide bond and (ii) a topologically favored catalytic triad with a continuous and deep groove. These structural features of A. oryzae cutinase are proposed to result in an improved hydrolytic activity and altered substrate specificity profile, enhanced thermostability, and remarkable reactivity toward the degradation of the synthetic polyester polycaprolactone. The results presented here provide insight into engineering new cutinase-inspired biocatalysts with tailor-made properties.
A Versatile Synthetic Platform for Discrete Oligo-and Polyesters Based on Optimized Protective Groups Via Iterative Exponential Growth
Duan, Suhua,Yang, Xiaojie,Yang, Ze,Liu, Yuxin,Shi, Qiunan,Yang, Zhilin,Wu, Haibing,Han, Yue,Wang, Yongquan,Shen, Hang,Huang, Zhihao,Dong, Xue-Hui,Zhang, Zhengbiao
, p. 10830 - 10837 (2021/12/02)
Discrete oligo-and polyesters are highly attractive for a wide range of investigations because of their great fidelity on structure-property relationships. Even though numerous synthetic approaches have been developed, a more versatile synthetic platform with broad group tolerance is still desired. Herein, we report an iterative exponential growth strategy for efficient synthesis of discrete oligo-and polyesters, employing an optimized protective group pair, namely, TBDPS ether and t-butyl ester. The versatility of the strategy is demonstrated by facile preparation of several structurally diverse discrete oligo-and polyesters under mild, safe, and scalable reaction conditions, with the number of repeat units up to 256. Moreover, the contributions of the terminal protective groups on the melting and crystallization behaviors of discrete oligo-and poly(?-caprolactone)s were investigated. With this robust synthetic platform, various functional moieties can be readily incorporated into polyester chains, and a myriad of applications of polyesters could thus be reasonably anticipated.
