668984-45-0Relevant academic research and scientific papers
A ratiometric fluorescent sensor for rapid detection of the pyroglutamate aminopeptidase-1 in mouse tumors
Cao, Ting,Iqbal, Anam,Iqbal, Kanwal,Liu, Yun,Qian, Jing,Qin, Wenwu,Zhang, Liang,Zheng, Lei
supporting information, p. 4546 - 4554 (2021/06/16)
Pyroglutamate aminopeptidase-1 (PGP-1) is an important enzyme that plays an indispensable role in the process of inflammation. Up to now, few reports have been reported on the detection of PGP-1 activityin vivoandin vitro, and there are no reports on ratiometric detection. Here, the first red-emitting ratiometric fluorescent sensor (DP-1) for the specific detection of PGP-1 bothin vivoandin vitrowas designed and synthesized by using DCD-NH2as the luminescent parent and pyroglutamate as a recognition group. After interacting with PGP-1, the amide bond is hydrolyzed by the enzyme and the color of the solution changes from yellow (λabs= 420 nm) to red (λabs= 520 nm), accompanied by obvious fluorescence emission wavelength change (from ～564 nm to ～616 nm). The probe has high specificity and sensitivity towards PGP-1 in about 10 min, and the DL is as low as 0.25 ng mL?1. Interestingly, under the stimulation of Freund's incomplete adjuvant and lipopolysaccharide, the imaging ofDP-1in HepG2 and RAW264 cells shows that the expression of PGP-1 is associated with inflammation. What's more, for the first, the imaging of a mouse tumor model confirms that the enzyme is closely related to the occurrence of some inflammation and tumor diseases. These results indicate thatDP-1can be used as an effective tool for real-time monitoring of PGP-1 levels bothin vivoandin vitroand the study of inflammatory tumor pathology.
Increase of tyrosinase activity at the wound site in zebrafish imaged by a new fluorescent probe
Chai, Ziyin,Shang, Jizhen,Shi, Wen,Li, Xiaohua,Ma, Huimin
, p. 2764 - 2767 (2021/03/22)
Tyrosinase plays a pivotal role in the hyperpigmentation of wounds. Here, we develop a new fluorescent probe and with it, we reveal an increase of tyrosinase activity at the wound site in zebrafish.
Red-Emitting Fluorescent Probe for Detection of γ-Glutamyltranspeptidase and Its Application of Real-Time Imaging under Oxidative Stress in Cells and in Vivo
Liu, Feiyan,Wang, Zhen,Wang, Wenli,Luo, Jian-Guang,Kong, Lingyi
, p. 7467 - 7473 (2018/05/30)
γ-Glutamyltranspeptidase (GGT) plays critical roles in regulating various physiological/pathophysiological processes including the intracellular redox homeostasis. However, an effective fluorescent probe for dissecting the relationships between GGT and oxidative stress in vivo remains largely unexplored. Herein, we present a light-up fluorescent probe (DCDHF-Glu) with long wavelength emission (613 nm) for the highly sensitive and selective detection of GGT using dicyanomethylenedihydrofuran derivative as the fluorescent reporter and γ-glutamyl group as the enzyme-active trigger. DCDHF-Glu is competent to real-time image endogenous GGT in live cells and mice. In particular, DCDHF-Glu enables the direct real-time visualization of the upregulation of GGT under drug-induced oxidative stress in the HepG2 cells and the LO2 cells, as well as in vivo, vividly implying its excellent capacity in elucidation of GGT function in GGT-related biological events.
A red emission fluorescent probe for hydrogen sulfide and its application in living cells imaging
Chen, Tao,Zheng, Yi,Xu, Zhaochao,Zhao, Miao,Xu, Yongnan,Cui, Jingnan
, p. 2980 - 2982 (2013/07/05)
Hydrogen sulfide has emerged as an important biological messenger and much attention has been paid to the design of fluorescent probes for H2S to meet the requirement of accurate measurement of H2S. In this work, a new red emission fluorescent probe for H2S was developed based on the reduction reaction of azide with H2S to amine with the fluorophore of dicyanomethylenedihydrofuran because of its long excitation and emission wavelength. The probe has a high selectivity for H2S over competitive anions and sulfide-containing analytes. Finally, the probe was applied to sense H2S in living cells.
Azido push-pull fluorogens photoactivate to produce bright fluorescent labels
Lord, Samuel J.,Lee, Hsiao-Lu D.,Samuel, Reichel,Weber, Ryan,Liu, Na,Conley, Nicholas R.,Thompson, Michael A.,Twieg, Robert J.,Moerner
, p. 14157 - 14167 (2011/04/24)
Dark azido push-pull chromophores have the ability to be photoactivated to produce bright fluorescent labels suitable for single-molecule imaging. Upon illumination, the aryl azide functionality in the fluorogens participates in a photochemical conversion to an aryl amine, thus restoring charge-transfer absorption and fluorescence. Previously, we reported that one compound, DCDHF-V-P-azide, was photoactivatable. Here, we demonstrate that the azide-to-amine photoactivation process is generally applicable to a variety of push-pull chromophores, and we characterize the photophysical parameters including photoconversion quantum yield, photostability, and turn-on ratio. Azido push-pull fluorogens provide a new class of photoactivatable singlemolecule probes for fluorescent labeling and super-resolution microscopy. Lastly, we demonstrate that photoactivated push-pull dyes can insert into bonds of nearby biomolecules, simultaneously forming a covalent bond and becoming fluorescent (fluorogenic photoaffinity labeling).
