68802-01-7

Upstream product

• 1152-61-0 N-Cbz-L-Asp 
• 7524-50-7 methyl (2S)-2-amino-3-phenylpropanoate hydrochloride 
• 4515-21-3 N-benzyloxycarbonylaspartic acid 
• 5619-07-8 methyl 2-amino-3-phenylpropanoate hydrochloride 
• 2577-90-4 methyl (2S)-2-amino-3-phenylpropanoate 

Downstream Products

• 33605-72-0 N-carbobenzyloxy-L-aspartyl-L-phenylalanine methyl ester 

Relevant academic research and scientific papers

Immobilized thermolysin and synthesis of precursor of aspartame

The optimal condition of the thermolysin-catalysed condensation reaction of N-benzyloxycarbonyl-DL-aspartic acid (Z-DL-Asp) with DL-phenylalanine methyl ester (DL-Phe-OMe) giving N-benzyloxycarbonyl-L-aspartyl-L-phenylalanine methyl ester, the precursor of the synthetic sweetener (aspartame, APM) has been investigated.The immobilized thermolysins have been prepared using seven polymethacrylate derivatives as carrier by covalent coupling of the polymers with enzyme.Seven derivatives have been synthesized using methacrylic acid, p-hydroxybenzoic acid and thecorresponding activated amide or amine.The abilities of the immobilized thermolysis to catalyze the condensation reaction have been tested and 100percent yield has been obtained by using one of them.

Enzymic synthesis design and enzymic synthesis of aspartame

An enzymic synthesis of aspartame (H-Asp-Phe-OMe) has been designed and realized based on the structure-activity study of thermolysin and penicillin amidase hydrolysis of its p-substituted phenylacetyl derivatives. These compounds meet the structural and energetic requirements of two enzymic binding sites. The peptide sweetener has been prepared by thermolysin - catalyzed condensation of the p-substituted phenylacetyl-Asp-OH and H-Phe-OMe follwed by penicillin amidase - catalyzed deprotection of the resulted aspartame precursors.