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Pentalenic acid is a sesquiterpenoid carboxylic acid that serves as a shunt metabolite in the biosynthesis of the pentalenolactone family of antibiotics, produced by *Streptomyces* species. It is derived from 1-deoxypentalenic acid through hydroxylation mediated by the cytochrome P450 enzyme CYP105D7 (SAV-7469) in *Streptomyces avermitilis*. This conversion occurs as part of a secondary metabolic pathway, where pentalenic acid accumulates alongside other related metabolites. Its biosynthesis involves oxidative modifications facilitated by CYP105D7, supported by ferredoxin and ferredoxin reductase, confirming its role as an intermediate rather than a primary end product in the pentalenolactone biosynthetic network.

69394-19-0

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69394-19-0 Usage

Check Digit Verification of cas no

The CAS Registry Mumber 69394-19-0 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 6,9,3,9 and 4 respectively; the second part has 2 digits, 1 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 69394-19:
(7*6)+(6*9)+(5*3)+(4*9)+(3*4)+(2*1)+(1*9)=170
170 % 10 = 0
So 69394-19-0 is a valid CAS Registry Number.

69394-19-0Downstream Products

69394-19-0Relevant academic research and scientific papers

Intramolecular Photocycloaddition-Cyclobutane Fragmentation: A Highly Stereoselective Total Synthesis of (+/-)-Pentalenic Acid

Crimmins, Michael T.,DeLoach, Joe A.

, p. 2076 - 2077 (1984)

An efficient total synthesis of pentalenic acid has been accomplished by utilizing an intramolecular photocycloaddition-cyclobutane fragmentation as the key reaction sequence.

SYNTHESIS OF PENTALENIC ACID THROUGH BIOGENETIC LIKE CYCLIZATION OF HUMULENE

Sakai, Kazuya,Ohtsuka, Toshikazu,Misumi, Shunjiro,Shirahama, Haruhisa,Matsumoto, Takeshi

, p. 355 - 358 (1981)

Humulene furnished 4,7-epoxy-3-methylene-7,10,10-trimethyl-11-bicycloundecanol 9 in 34percent yield employing oxymercuration as a key step.On treatment with BF3.OEt2, 7,11-dihydroxy-3,7,10,10-tetramethyl-3-bicycloundecene, which was derived from 9 by ether cleavage, afforded 10α-hydroxy-pentalenene 13 (20percent) along with four byproducts.Oxidation of allylic methyl group of 13 gave methyl pentalenate in 13percent yield from 9.

Pentalenic acid is a shunt metabolite in the biosynthesis of the pentalenolactone family of metabolites: Hydroxylation of 1-deoxypentalenic acid mediated by CYP105D7 (SAV-7469) of Streptomyces avermitilis

Takamatsu, Satoshi,Xu, Lian-Hua,Fushinobu, Shinya,Shoun, Hirofumi,Komatsu, Mamoru,Cane, David E,Ikeda, Haruo

, p. 65 - 71 (2011)

Pentalenic acid (1) has been isolated from many Streptomyces sp. as a co-metabolite of the sesquiterpenoid antibiotic pentalenolactone and related natural products. We have previously reported the identification of a 13.4-kb gene cluster in the genome of Streptomyces avermitilis implicated in the biosynthesis of the pentalenolactone family of metabolites consisting of 13 open reading frames. Detailed molecular genetic and biochemical studies have revealed that at least seven genes are involved in the biosynthesis of the newly discovered metabolites, neopentalenoketolactone, but no gene specifically dedicated to the formation of pentalenic acid (1) was evident in the same gene cluster. The wild-type strain of S. avermitilis, as well as its derivatives, mainly produce pentalenic acid (1), together with neopentalenoketolactone (9). Disruption of the sav7469 gene encoding a cytochrome P450 (CYP105D7), members of which class are associated with the hydroxylation of many structurally different compounds, abolished the production of pentalenic acid (1). The sav7469-deletion mutant derived from SUKA11 carrying pKU462-ptl- clusterΔptlH accumulated 1-deoxypentalenic acid (5), but not pentalenic acid (1). Reintroduction of an extra-copy of the sav7469 gene to SUKA11 Δsav7469 carrying pKU462-ptl-clusterΔptlH restored the production of pentalenic acid (1). Recombinant CYP105D7 prepared from Escherichia coli catalyzed the oxidative conversion of 1-deoxypentalenic acid (5) to pentalenic acid (1) in the presence of the electron-transport partners, ferredoxin (Fdx) and Fdx reductase, both in vivo and in vitro. These results unambiguously demonstrate that CYP105D7 is responsible for the conversion of 1-deoxypentalenic acid (5) to pentalenic acid (1), a shunt product in the biosynthesis of the pentalenolactone family of metabolites.

Intramolecular Photocycloadditions-Cyclobutane Fragmentation: Total Synthesis of (+/-)-Pentalenene, (+/-)-Pentalenic Acid, and (+/-)-Deoxypentalenic Acid

Crimmins, Michael T.,DeLoach, Joe A.

, p. 800 - 806 (2007/10/02)

Pentalenene, pentalenic acid, and deoxypentalenic acid, important metabolites in the biosynthesis of the pentalenolactones, have been synthesized from methyl isobutyrate through a common intermediate.The initial key stp involves a novel conjugate addition

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