7008-42-6 Usage
Originator
Acronine ,ZYF Pharm Chemical
Uses
Antineoplastic.
Definition
ChEBI: An alkaloid antineoplastic agent isolated from Acronychia baueri.
Manufacturing Process
The acridone alkaloids constitute a small group of natural products found
exclusively in the Rutaceae family of higher plants. A sustained interest in this
field has been due to the reported activity of acronycine a constituent of
Acronnychia baueri and Vepris amphody as an anti-tumor agent.
There are different methods of the synthetic preparation of acronycine (W. M.
Bandaranayake et al., J. Chem. Soc. Perkin 1, 998 (1968); J. Hlubucek et al.;
Aust. J. Chem. 23, 1881 (1970). One of them is described below. Friedel-
Crafts condensation between 2-nitrobenzoyl chloride and 3,5-
dimethoxyphenol.
2-Nitrobenzoyl chloride (12 g) and AlCl3, (anhyd., 13 g) were dissolved in dry
ether (50 ml) and this mixture added to a solution of 3.5-dimethoxyphenol (5
g) in dry ether 150 ml) at 0°C and the final mixture stirred at 0°C for 3
hours, brought to 20°C and stirred for a further 3 h. Diluted HCl and ice were
added and the product extracted with EtOAc (3x50 ml), this extract was
washed with aq. NaHCO3, water, dried (MgSO4), filtered and evaporated under
reduced pressure to yield a dark red oil. This oil was treated with 2 M aq.
NaOH. (100 ml) for 1 h., acidified with diluted HCl and re-extracted with EtOAc which gave, after a similar work up, a paled red oil (5.0 g). Thin layer
chromatography (TLC) showed three components one of which was the
starting phenol. Column chromatography (150 g silica gel) and elution with
benzene:petrol ether 40°-60°C (1:1) followed by increasing polarity of
solvents (benzene through chloroform to chloroform:ether (4:1) gave 59
fractions. Fractions 1-12 were combined to gave a solid, which crystallized
from benzene to give 4,6-dimethoxy-2-hydroxy-2-nitrobenzophenone, MP:
198°-199°C. Fractions 13-21 were discarded. Fractions 22-42 were combined
(0.3 g), crystallized from benzene and the product added to that obtained
from fractions 43-59 which crystallized from benzene to give 2,6-dimethoxy-
7-hydroxy-2-nitrobenzophenone (0.5 g) MP: 175°-177°C.
Condensation of 3-chloro-3-methylbutyne with 2,6-dimethoxy-7-hydroxy-2-
nitrobenzophenone:
A solution of the above benzophenone (2 g) and excess 3-chloro-3-
methylbutyne (4.5 g) in dry DMF (60 ml) containing anhydrous K2CO3 (4 g)
and dry KI (2 g) was stirred and heated at 65°C for 14 hours (under N2). The
mixture was cooled, diluted with water, acidified, extracted with chloroform
(3x50 ml) and the extract was worked up in the usual way (including a NaOH
wash) to give an oil, which was redissolved in DMF (20 ml) and heated at
130°C, under N2, for 7 h whence most of the starting material had
disappeared. The solvent was removed under reduced pressure to give a
product (0.62 g) which was purified by preparative layer chromatography on
silica gel to give 6-(2-nitrobehzoyl)-5,7-dimethoxy-2,2-dimethylchromene
(0.22 g) which crystallized from EtOH, MP: 92°-93°C.
6-(2-Aminobenzoyl)-5,7-dimethoxy-2,2-dimethylchromene (0.2 g) was
dissolved in EtOH (30 ml) containing water (5 ml) and ammonium chloride (1
g) and Zn mossy (1.5 g) was added in portions and the mixture stirred at
room temperature for 5 days. The solution was filtered, evaporated to dryness
under reduced pressure and the residue dissolved in EtOAc (25 ml) and
worked up in the usual way to give a solid (0.19 g). It crystallised from EtOH
with MP: 123°-126°C.
Cyclization of aminodimethylchromenylbenzophenone: 6-(2-aminobenzoyl)-
5,7-dimethoxy-2,2-dimethylchromene (0.12 g) was dissolved in DMSO (8 ml)
and NaH (0.06 g) added, the mixture was stirred for 6 days at room
temperature. A further addition of NaH (0.06 g) was made and the solution
heated to 50°C for 0.5 h whence it was poured into water, extracted with
EtOAc and worked up in the usual way to give a crude mixture (0.11 g;
components). Separation of this mixture on plate (silica gel:benzene:EtOAc,
10:4) gave band 1 (Rf 0.45: 38 mg) identified as starting material. Band 2 (Rf
0.32: 42 mg; 43%) which crystallized from ethylacetate as des-Nmethylisoacronycine,
MP: 293°-295°C. Band 3 (Rf 0.10; 29 mg, 29%)
crystallized from ethyl acetate as des-N-methylacronycine. MP: 237°-240°C.
Des-N-methylacronycine (14 mg) was dissolved in dry acetone (10 ml),
anhydrous K2CO3 (1 g), and MeI (2 ml) added and the mixture refluxed for 11
hours. The solution was filtered and the solvents evaporated to give a solid
(12 mg) which after purification on TLC, gave acronycine which crystallized
from aqueous MeOH, MP: 171°-173°C. This product showed identical U.V. and
Rf characterization when compared with acronycine and had an accurate mass measurement of 321.1368. C20H19NO3, required: 321.1364. UV and 1H NMR
spectrum confirmed the structures of all described compounds.
Therapeutic Function
Antineoplastic
General Description
Yellow powder.
Air & Water Reactions
Water insoluble.
Reactivity Profile
Amines, like Acronine, are weak chemical bases. They neutralize acids to form salts plus water. These acid-base reactions are exothermic. The amount of heat that is evolved per mole of amine in a neutralization is largely independent of the strength of the amine as a base. Amines may be incompatible with isocyanates, halogenated organics, peroxides, phenols (acidic), epoxides, anhydrides, and acid halides. Flammable gaseous hydrogen is generated by amines in combination with strong reducing agents, such as hydrides.
Health Hazard
ACUTE/CHRONIC HAZARDS: When heated to decomposition Acronine emits toxic fumes.
Fire Hazard
Flash point data for Acronine are not available. Acronine is probably combustible.
Check Digit Verification of cas no
The CAS Registry Mumber 7008-42-6 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 7,0,0 and 8 respectively; the second part has 2 digits, 4 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 7008-42:
(6*7)+(5*0)+(4*0)+(3*8)+(2*4)+(1*2)=76
76 % 10 = 6
So 7008-42-6 is a valid CAS Registry Number.
InChI:InChI=1/C20H21NO3/c1-20(2)10-9-13-15(24-20)11-16(23-4)17-18(13)21(3)14-8-6-5-7-12(14)19(17)22/h5-11,19,22H,1-4H3
7008-42-6Relevant articles and documents
Total synthesis of acronycine and noracronycine: An aryne amination approach
Xu, Yuan-Ze,Wen, Qi-Ling,Sha, Feng,Li, Qiong,Wu, Xin-Yan
, (2021/06/28)
Acronycine and noracronycine are chromene-containing alkaloids with significant biological activity. We have accomplished a concise total synthesis of acronycine and noracronycine. The key step, regioselective nucleophilic addition of anthranilate to chromene-type arynes under mild and transition-metal-free conditions was achieved. In addition, further modifications of nucleophilic addition products, such as hydrogenation, O-functionalization and palladium-catalyzed coupling reactions have also been developed, providing a concise procedure for these alkaloids and their derivatives.
A practical regiospecific approach towards acronycine and related alkaloids
Anand,Selvapalam
, p. 199 - 200 (2007/10/03)
Highly regiospecific prenylation of 3,5-dimethoxyacetanilide and cyclization of 2-[3,5-dimethoxy-2(3-methylbut-2-enyl)]aminobenzoic acid 6 under mild conditions in high yields provides a practical and flexible synthesis of acronycine, glycocitrine-II and
Synthesis of des-N-methylacronycine and acronycine
Loughhead
, p. 2245 - 2246 (2007/10/02)
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