71-63-6 Usage
Description
Different sources of media describe the Description of 71-63-6 differently. You can refer to the following data:
1. Digitoxin is the most powerful and reliable of the glucosides which have been extracted from digitalis leave. It is a cardiac glycoside (CG). It is a cardiotonic drug, which exhibits cardiac and anti-cancer properties. Digitoxin inhibits nuclear factor kappa B (NF-κB) signaling. It is used to treat congestive heart failure and cardiac arrhythmia. Digitoxin prevents microtubule formation.
2. Digitoxin is a cardiac glycoside that has been found in Digitalis and has diverse biological activities. It inhibits human recombinant α1β1, α2β2, and α3β1 subunit-containing Na+/K+-ATPases with Ki values of 250, 63, and 136 nM, respectively. Digitoxin inhibits the human-ether-a-go-go (hERG) potassium channel, also known as Kv11.1, in HEK293 cells expressing hERG (IC50 = 11.1 nM). It enhances developed tension and contractile force in electrically stimulated isolated guinea pig left atrial muscle when used at concentrations of 0.2 and 0.4 μM, respectively. Dietary administration of digitoxin (~1 mg/kg per day) attenuates congestive heart failure and reduces myocardial hypertrophy in a rat model of myocardial infarction induced by coronary artery ligation. Digitoxin is also cytotoxic to a panel of 10 human cancer cell lines, including myeloma, lymphoma, and leukemia cancer cells, with IC50 values ranging from 12 to 76 nM. Formulations containing digitoxin have previously been used in the treatment of congestive heart failure and cardiac arrhythmias.
Chemical Properties
White Solid
Originator
Crystodigin,Lilly
Uses
Different sources of media describe the Uses of 71-63-6 differently. You can refer to the following data:
1. Digitoxin is used for chronic cardiac insufficiency, tachyarrhythmia form of atrial fibrillation,
paroxysmal ciliary arrhythmia, and paroxysmal supraventricular tachycaria.
2. Digitoxin has been used as a labeled drugs for binding sites on human serum albumin (HSA). It has also been used to test its anti-transmissible gastroenteritis virus (TGEV) activity.
Indications
Digitoxin (Dig) is an FDA approved drug for the treatment of cardiac disease.Digitoxin is used for the treatment of heart failure, especially in people with impaired kidney function. It is also used to treat certain kinds of heart arrhythmia, such as atrial fibrillation.Digoxin is used to treat heart failure and abnormal heart rhythms (arrhythmias). It also helps the heart work better and control heart rate. Digoxin may be used after a heart attack. This medication comes in various forms: tablet, capsule, or pediatric elixir (liquid). It is available under the brand names Lanoxin, Cardoxin, Digitek, Digox, and Lanoxicaps.
Manufacturing Process
1000 g of Digitalis purpurea leaves were moistened thoroughly with a
menstruum consisting of 60% ethyl alcohol and 40% water and were packed
in a percolator with enough of the menstruum to leave a stratum above the
drug. After maceration overnight, the drug was percolated with about 7 liters of the 60%-alcohol menstruum and about 5 liters of percolate or extract were
collected. 400 g of solid lead acetate were added to the percolate and the
mixture was stirred until all the lead acetate had dissolved. After standing for
at least one hour, the copious light green precipitate was centrifuged off and
washed successively with 1000 ml and 500 ml portions of 60% alcohol. The
washings were combined with the filtrate from the centrifuge and most of the
excess lead acetate removed by treatment with a saturated solution of sodium
carbonate monohydrate. The resulting lead carbonate was filtered off, washed
with two 200 ml portions of 60% ethyl alcohol, and the washings combined
with the filtrate. Hydrogen sulfide was then passed through the combined
liquids until no more lead sulfide precipitated. The filtrate and washings
resulting from filtering off the lead sulfide were concentrated in vacuo at or
below 40°C to a volume of 2000 ml and saturated with a salt, such as sodium
chloride, to facilitate subsequent extraction with a water-immiscible organic
solvent. The mixture was extracted five times with 600 ml of a solvent
consisting of two volumes of chloroform and three volumes of amyl ether. The
chloroform-amyl ether solution is extracted with about four 400 ml portions of
a 10% solution of sodium carbonate monohydrate to remove any gitalin that
may have carried through in the process and vegetative extractive material.
After drying over anhydrous sodium sulfate and filtering, the chloroform-amyl
ether solution was concentrated in vacuo at 75°-85°C to a volume of about 25
ml. After cooling to room temperature, the concentrate was mixed with about
four volumes of petroleum ether and allowed to stand for about one hour at
room temperature. The dark colored, amorphous precipitate was filtered and
washed with petroleum ether to ensure that all fat had been removed. The
precipitate was dissolved in 100 ml of dilute alcohol (1:1) and the slight
precipitate remaining after thorough agitation was filtered off. The filtrate was
made slightly alkaline with 10% ammonia water and 10 g of solid lead acetate
were dissolved therein with agitation. The light brown precipitate, which
formed, was centrifuged off and washed with two 50 ml portions of dilute
alcohol. Excess lead acetate was removed by passage of hydrogen sulfide
through the solution until no more lead sulfide precipitated. The filtrate and
washings resulting from removal of the lead sulfide was concentrated below
40°C. After making slightly alkaline with ammonia water, the concentrate was
extracted with three 50 ml portions of chloroform. The chloroform extract of
digitoxin was dried over anhydrous sodium sulfate. After filtering and washing
the filter with dry chloroform, the chloroform extract was heated on a water
bath to remove the chloroform and the residue was dissolved in 20ml of hot
alcohol at about 60°C., and diluted with hot distilled water at 60°C to an
alcohol concentration of 30%. Upon standing overnight, the digitoxin settled
out as a yellowish orange, mostly amorphous solid together with some needle
and rosette crystals.The digitoxin was filtered off, and dried in a vacuum desiccator over calcium
chloride and then was dissolved In 10 cc. of dry chloroform after which 15 ml
of dry amyl ether was added, followed by 100 ml of petroleum ether. After
standing one hour, the precipitate was filtered off, washed with petroleum
ether, and dried in a vacuum desiccator until all traces of amyl ether were
removed. One 1ml of alcohol for each 25 milligrams of material was added to
the dried precipitate and the mixture was heated on a water-bath at 60°C
until the precipitate had completely dissolved, after which hot distilled water
at 60°C was added to produce an alcohol concentration of 40%. Upon
standing overnight at room temperature the digitoxin came down as almost
completely white crystals. Upon recrystallizing a second time from 40%
alcohol, completely white crystals of digitoxin were obtained. On the basis of the digitalis cat assay, the digitoxin was completely pure and is a prompt and
powerful heart tonic in doses of 25 mg to 1 mg. The crystalline digitoxin is
also substantially stable and may be relied upon by the physician to furnish a
uniform degree of activity of the same kind insofar as the digitoxin is
concerned.
Brand name
Crystodigin (Lilly)
.
Therapeutic Function
Cardiotonic, Topical venotonic
General Description
Odorless white or pale buff microcrystalline powder. Used as a cardiotonic drug.
Health Hazard
Material is bioactive and capable of causing cardiac arrythmias and electrolyte imbalances that may be fatal. Death is due to ventricular fibrillation or cardiac standstill. Material has a high toxicity hazard rating; it may cause death or permanent injury after a very short exposure. It is classified as super toxic; an estimated single lethal dose is 3-10 mg.
Fire Hazard
When heated to decomposition, DIGITOXIN emits acrid smoke and irritating fumes.
Safety Profile
A deadly poison by
most routes. Human systemic effects:
arrhythmias, cardiomyopathy, EKG
changes, nausea or vomiting, paresthesia,
pulse rate increase, thrombocytopenia.
Human reproductive effects by ingestion:
reduced viability of newborn. An eye irritant. When heated to decomposition it
emits acrid smoke and irritating fumes. See
also DIGITALIS.
Purification Methods
Digitoxin crystallises from MeOH, aqueous EtOH with 0.5 to 1 H2O and from H2O as the dihydrate. It also crystallises from CHCl3/Et2O as anhydrous crystals. The hydrate dehydrates at 120o/vacuum. Its solubility is 2.5% in CHCl3, 1.7% in EtOH, 0.25% in EtOAc, and 0.001% in H2O; and has E1cm 202.5 at 219-220nm (50% EtOH). [Stoll et al. Helv Chim Acta 37 1134 1954, Demoen & Janssen J Am Pharm Assoc 42 635 1953, Beilstein 18 IV 1478.]
Mode of action
Digitoxin is a purified cardiac glycoside similar in structure and function to Digoxin. Unlike Digoxin, Digitoxin is eliminated from the body via the liver and not the kidneys. Both drugs are used to treat various heart conditions. Cardiac glycosides bind to a site on the extracellular aspect of the a-subunit of the Na+/K+ ATPase pump in the membranes of heart cells (myocytes). This causes an increase in the level of sodium ions in the myocytes, which then leads to a rise in the level of calcium ions. Digitoxin inhibits the sodium-potassium ATPase in heart muscle cells, resulting in increased force of contractions (positive inotropic), reduced speed of electric conduction (negative dromotropic), increased excitability (positive bathmotropic), and reduced frequency of heartbeat (negative chronotropic).
Check Digit Verification of cas no
The CAS Registry Mumber 71-63-6 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 7 and 1 respectively; the second part has 2 digits, 6 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 71-63:
(4*7)+(3*1)+(2*6)+(1*3)=46
46 % 10 = 6
So 71-63-6 is a valid CAS Registry Number.
InChI:InChI=1/C41H64O13/c1-20-36(46)29(42)16-34(49-20)53-38-22(3)51-35(18-31(38)44)54-37-21(2)50-33(17-30(37)43)52-25-8-11-39(4)24(15-25)6-7-28-27(39)9-12-40(5)26(10-13-41(28,40)47)23-14-32(45)48-19-23/h14,20-22,24-31,33-38,42-44,46-47H,6-13,15-19H2,1-5H3/t20-,21-,22-,24-,25+,26-,27?,28?,29+,30+,31+,33+,34+,35+,36-,37-,38-,39+,40-,41+/m1/s1
71-63-6Relevant articles and documents
Catalytic stereoselective synthesis of β-digitoxosides: Direct synthesis of digitoxin and C1′-epi-digitoxin
Baryal, Kedar N.,Adhikari, Surya,Zhu, Jianglong
, p. 12469 - 12476 (2014/01/17)
A mild and atom-economic rhenium(V)-catalyzed stereoselective synthesis of β-d-digitoxosides from 6-deoxy-d-allals has been described. This β-selective glycosylation was achieved probably because of the formation of corresponding α-digitoxosides disfavored by 1,3-diaxial interaction. In addition, this method has been successfully applied to the synthesis of digitoxin trisaccharide glycal for the direct synthesis of digitoxin and C1′-epi-digitoxin.
A direct comparison of the anticancer activities of digitoxin MeON-Neoglycosides and O-Glycosides
Iyer, Anand Krishnan V.,Zhou, Maoquan,Azad, Neelam,Elbaz, Hosam,Wang, Leo,Rogalsky, Derek K.,Rojanasakul, Yon,O'Doherty, George A.,Langenhan, Joseph M.
scheme or table, p. 326 - 330 (2010/12/18)
Digitoxin is a cardiac glycoside currently being investigated for potential use in oncology; however, an investigation of anticancer activity as a function of oligosaccharide chain length has not yet been performed. We generated mono-, di-, and tri-O-digitoxoside derivatives of digitoxin and compared their activities to the corresponding MeON-neoglycosides. Both classes of cardenolide derivatives display comparable oligosaccharide chain length-dependent cytotoxicity toward human cancer cell lines. Further investigation revealed that both classes of compounds induce caspase-9-mediated apoptosis in non-small cell lung cancer cells (NCI-H460). Because O-glycosides and MeON-neoglycosides share a similar mode of action, the convenience of MeON-neoglycosylation could be exploited in future SAR work to rapidly survey large numbers of carbohydrates to prioritize selected O-glycoside candidates for traditional synthesis.
De novo approach to 2-deoxy-β-glycosides: Asymmetric syntheses of digoxose and digitoxin
Zhou, Maoquan,O'Doherty, George A.
, p. 2485 - 2493 (2008/02/02)
A highly enantioselective and straightforward route to trisaccharide natural products digoxose and digitoxin has been developed. Key to this approach is the iterative application of the palladium-catalyzed glycosylation reaction, reductive 1,3-transposition, diastereoselective dihydroxylation, and regioselective protection. The first total synthesis of natural product digoxose was accomplished in 19 total steps from achiral 2-acylfuran, and digitoxin was fashioned in 15 steps starting from digitoxigenin 2 and pyranone 8β. This flexible synthetic strategy also allows for the preparation of mono- and disaccharide analogues of digoxose and digitoxin.
