71100-71-5

Upstream product

• 68892-40-0 N⁴-anisoyl-5'-O-(4,4'-dimethoxytrityl) 2'-deoxy cytidine 
• 109-78-4 3-Hydroxypropionitrile 
• 772-79-2 4-chlorophenylphosphorodichloridate 
• 32666-92-5 p-chlorophenyl phosphorodichloridite 

Relevant academic research and scientific papers

PREPARATION RAPIDE DES MONONUCLEOSIDES-3' MONOPHOSPHATE PROTEGES UTILISABLES EN SYNTHESE OLIGONUCLEOTIDIQUE

Fully protected mononucleotides are prepared by the action of parachlorophenylphosphodichloridite in THF on the corresponding nucleosides followed by oxidation with iodine.

Nucleosides and Nucleotides. Part 17. A Simple Preparation of Protected Deoxynucleoside-3'-phosphates

A simple method for the preparation of fully protected 2'-deoxynucleoside-3'-phosphates is described.The main step, phosphorylation of partially protected deoxynucleosides, is performed with the monofunctional reagent p-chlorophenyl(2-cyanoethyl)phosphochloridate (2) in dioxane.This reagent is quickly prepared from readily accessible p-chlorophenyl phosphodichloridate by reaction with 3-hydroxypropionitrile and triethylamine in dioxane or ether.The crude reagent is used directly for phosphorylation.After chromatography, protected deoxynucleoside-3'-phosphonates were isolated in yields of 80-87percent .The method described here, which has been optimized in detail, represents a simple alternative to published methods.