7208-41-5Relevant academic research and scientific papers
Isolation and characterization of a hyperbranched proteoglycan from Ganoderma Lucidum for anti-diabetes
Pan, Deng,Wang, Linqiang,Chen, Congheng,Hu, Bingwen,Zhou, Ping
, p. 106 - 114 (2015)
Presently, an efficient protein tyrosine phosphatase 1B (PTP1B) inhibitor, named FYGL-n, was isolatedfrom Ganoderma Lucidum and characterized for its structure and bioactivity. Structure and chain con-formation of FYGL-n based on both chemical and spectroscopic analysis showed that FYGL-n was ahyperbranched heteropolysaccharide bonded with protein via both serine and threonine residues by O-type glycoside, and showed a sphere observed by AFM. Specifically, monosaccharide compositionindicated that FYGL-n consisted of D-arabinose, D-galactose, L-rhamnose and D-glucose in a mole ratio of 0.08:0.21:0.24:0.47, with a molecular mass of 72.9 kDa. The analysis of amino acids in FYGL-n indicatedthat there were 16 common amino acids, among which aspartic acid, glycine, serine, alanine, glutamicacid and threonine were the dominant components. Also it was demonstrated that FYGL-n could inhibitthe PTP1B activity on a competitive mechanism in vitro.
Isolation, purification and structural characterization of a water-soluble polysaccharide HM41 from Halenia elliptica D. Don
Liu, Chun-Lan,Li, Yang,Xu, Gui-Yun,Li, Ya-Shuang
, p. 979 - 983 (2016/06/13)
A water-soluble polysaccharide, HM41, was obtained from Halenia elliptica D. Don by acidic ethanol fractionation and gel filtration. Its homogeneity was confirmed by chromatography using multiple systems. HM41 was composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal), glucose (Glc) with a molar ratio of 1.0:5.5:1.8:3.0:9.4:21. The average molecular weight of HM41 was approximately 1.17 × 104. Periodate oxidation, Smith degradation, methylation and GC, IR, NMR, XRD, GC-MS analysis were used for the structural analysis of HM41. Its main chain was composed mainly of β-(1 → 4)Gal, β-(1 → 4)Glc and β-(1 → 6)Glc. β-(1 → 4)Gal were substituted at 6-O and on average there were 14 branches among 23 main chain residues; (1 → 4)Glc had no branch; (1 → 6)Glc were substituted at 3-O and on average there were 9 branches among 14 main chain residues. The side chain was composed of (1 → 3,6)-Rha, (1 → 4)/(1 → 5)-Ara, (1 → 4)/(1 → 5)-Xyl, (1 → 4,6)-Man and (1 → 2)-Glc. The terminal residue was composed of Ara, Xyl, Man, Gal, and Glc. Then, we demonstrated that HM and HM41 had strong scavenging activities in vitro hydroxyl. Overall, HM and HM41 may have potential applications in the antioxidants for medical and food industry.
CHARACTERIZATION OF 6-DEOXY-D-ALTRITOL IN THE CELL-WALL POLYSACCHARIDE OF Nocardia asteroides R 399
Voiland, Annie,Michel, Georges
, p. 283 - 288 (2007/10/02)
A polyol, found in the cell-wall of Nocardia asteroides R 399 as a component of a neutral polysaccharide mainly composed of D-arabinose and D-galactose, was identified by mass spectrometry, paper chromatography, thin-layer chromatography, and gas chromatography as 6-deoxy-D-altritol.
