72088-94-9 Usage
Description
5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE , 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability. 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers.
Chemical Properties
Dark-Orange to Brown Solid
Uses
Different sources of media describe the Uses of 72088-94-9 differently. You can refer to the following data:
1. A popular probe of determination of intracellular pH.
2. 5(6)-FAM, A useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent.
3. As a popular probe of determination of intracellular pH, 5(6)-Carboxyfluoresceincan be used as useful reagent for the preparation of hydrolytically stable fluorescent conjugates and a useful starting material for the synthesis of other fluorescein-derives reagent
Check Digit Verification of cas no
The CAS Registry Mumber 72088-94-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 7,2,0,8 and 8 respectively; the second part has 2 digits, 9 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 72088-94:
(7*7)+(6*2)+(5*0)+(4*8)+(3*8)+(2*9)+(1*4)=139
139 % 10 = 9
So 72088-94-9 is a valid CAS Registry Number.
