5(6)-Carboxyfluorescein

Base Information

• Chemical Name: 5(6)-Carboxyfluorescein
• CAS No.: 72088-94-9
• Molecular Formula: C21H12 O7
• Molecular Weight: 376.32
• Hs Code.: 29322090
• Mol file: 72088-94-9.mol

Synonyms:5(6)-Carboxyfluorescein;C 194; Carboxyfluorescein; Fluoresceincarboxylic acid

Chemical Property of 5(6)-Carboxyfluorescein

Chemical Property:

• Appearance/Colour: Off-white solid.
• Melting Point: 275 ºC
• Refractive Index: 1.6380 (estimate)
• Boiling Point: 464.9°C (rough estimate)
• PSA: 226.58000
• Density: 1.3418 (rough estimate)
• LogP: 6.72800
• Storage Temp.: Store at 2-8
• Solubility.: DMSO (Sparingly), Methanol (Slightly, Heated)
• Water Solubility.: slightly soluble

Purity/Quality:

99.9% ^*data from raw suppliers

5(6)-Carboxyfluorescein ^*data from reagent suppliers

Safty Information:

• Hazard Codes: Xi,Xn
• Statements: 36-36/37/38-20/21/22
• Safety Statements: 37/39-26-36-22

MSDS Files:

SDS file from LookChem

Useful:

• Description: 5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE , 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability. 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers.
• Uses: A popular probe of determination of intracellular pH. 5(6)-FAM, A useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent. As a popular probe of determination of intracellular pH, 5(6)-Carboxyfluoresceincan be used as useful reagent for the preparation of hydrolytically stable fluorescent conjugates and a useful starting material for the synthesis of other fluorescein-derives reagent

Refernces

Supramolecular aggregates of azobenzene phospholipids and related compounds in bilayer assemblies and other microheterogeneous media: Structure, properties, and photoreactivity

10.1021/ja971291n

The study focuses on the synthesis and investigation of azobenzene phospholipids (APLs) in aqueous dispersions, both in pure form and when mixed with saturated and unsaturated phospholipids. The research explores the structures of the assemblies formed by these APLs, which include various forms such as large plates, and their ability to form "H" aggregates with typical aggregation numbers being multiples of three. The study utilizes techniques like microcalorimetry, dynamic light scattering, cryo-transmission electron microscopy, and reagent entrapment to analyze the assemblies. It also examines the photoreactivity of the azobenzenes, which can photoisomerize to produce cis-rich photostationary states. Interestingly, the cis-azobenzenes do not aggregate and can be reverted back to the trans form through irradiation or thermal means. The research further explores the controlled release of entrapped reagents from vesicles formed by mixed aqueous dispersions of trans-APLs with other phospholipids, demonstrating that photoisomerization can induce reagent release. The study provides insights into how aggregation influences the microstructure and macroscopic properties of the assemblies, with potential applications in drug delivery and other areas requiring photoresponsive materials.