722-25-8

Basic information

• Product Name: 4-(p-tolylazo)aniline
• Synonyms: 4-(p-tolylazo)aniline;4-[(4-Methylphenyl)azo]benzenamine;4'-Methylazobenzene-4-amine;p-(p-Tolylazo)aniline
• CAS NO: 722-25-8
• Molecular Formula: C₁₃H₁₃N₃
• Molecular Weight: 211.26242
• EINECS: 211-960-7
• Mol File: 722-25-8.mol
• Article Data: 4

Chemical Properties

• Boiling Point: 340.95°C (rough estimate)
• Flash Point: 181.3°C
• Appearance: /
• Density: 1.1536 (rough estimate)
• Vapor Pressure: 7.43E-06mmHg at 25°C
• Refractive Index: 1.6000 (estimate)
• CAS DataBase Reference: 4-(p-tolylazo)aniline(CAS DataBase Reference)
• NIST Chemistry Reference: 4-(p-tolylazo)aniline(722-25-8)
• EPA Substance Registry System: 4-(p-tolylazo)aniline(722-25-8)

Safety Data

• Hazardous Substances Data: 722-25-8(Hazardous Substances Data)

Usage

InChI:InChI=1/C13H13N3/c1-10-2-6-12(7-3-10)15-16-13-8-4-11(14)5-9-13/h2-9H,14H2,1H3/b16-15+

Upstream product

• 142-04-1 aniline hydrochloride 
• 62-53-3 aniline 
• 622-74-2 N-phenyl-N'-p-tolyl-triazene 
• 785-86-4 1,3-di(p-tolyl)triaz-1-ene 
• 26021-90-9 sodium anilinomethanesulfonate 
• 106-49-0 p-toluidine 
• 100-01-6 4-nitro-aniline 
• 623-11-0 1-methyl-4-nitrosobenzene 
• 106-50-3 1,4-phenylenediamine 
• 115501-97-8 iminophenylenediamine 
• 67661-55-6 4-(N-acetylamino)-1-nitrosobezene 

Downstream Products

• 1119750-40-1 2-acetyl-2-ethoxycarbonyl-1-[4-(4-methylphenylazo)phenyl]-3-(4-dimethylaminophenyl)aziridine 
• 861519-21-3 N²-(4-iodo-phenyl)-4-methyl-o-phenylenediamine 
• 861324-42-7 N-(4-iodo-phenyl)-N'-p-tolyl-hydrazine 
• 28197-95-7 1-(4-iodo-phenyl)-6-methyl-1,3-dihydro-benzimidazole-2-thione 
• 949-87-1 4-Methylazobenzol 

Relevant articles and documents

Effect of Malt-PEG-Abz@RSL3 micelles on HepG2 cells based on NADPH depletion and GPX4 inhibition in ferroptosis

Ferroptosis is a regulated cell death pathway which depends on iron. Ferroptosis can be induced by limiting intracellular glutathione (GSH) synthesis, or inhibiting the activity of GPX4, or increasing intracellular accumulation of PE-AA-OOH, all of which involve NADPH. Therefore, NADPH depletion, excessive PE-AA-OOH, and GPX4 deficiency are generally considered to be the main characteristics of ferroptosis. In this research, the novel self-assembly nanomicelles modified by maltose ligand (Malt-PEG-Abz@RSL3) with superior nano characteristics were designed and fabricated. Malt-PEG-Abz@RSL3 micelles achieved active targeted drug delivery due to the high expression of glucose transporter (GLUT) and high uptake by HepG2 cells. Maltose-polyethylene glycol broke to release RSL3 for inhibiting GPX4 activity when Malt-PEG-Abz@RSL3 micelles entered the cells. Meanwhile, key coenzyme NADPH that participated in synthesis of GSH and Trx(SH)2 was depleted by azobenzene moiety, resulting in decreasing GSH and Trx(SH)2, which dually induced ferroptosis in tumour cells and promoted cell apoptosis.

Rapid and direct spectrophotometric method for kinetics studies and routine assay of peroxidase based on aniline diazo substrates

Peroxidases are ubiquitous enzymes that play an important role in living organisms. Current spectrophotometrically based peroxidase assay methods are based on the production of chromophoric substances at the end of the enzymatic reaction. The ambiguity regarding the formation and identity of the final chromophoric product and its possible reactions with other molecules have raised concerns about the accuracy of these methods. This can be of serious concern in inhibition studies. A novel spectrophotometric assay for peroxidase, based on direct measurement of a soluble aniline diazo substrate, is introduced. In addition to the routine assays, this method can be used in comprehensive kinetics studies. 4-[(4-Sulfophenyl)azo]aniline (λ max = 390 nm, ? = 32 880 M?1 cm?1 at pH 4.5 to 9) was introduced for routine assay of peroxidase. This compound is commercially available and is indexed as a food dye. Using this method, a detection limit of 0.05 nmol mL?1 was achieved for peroxidase.