72298-75-0Relevant academic research and scientific papers
Sequence specificity of hydrogen-bonded molecular duplexes
Zeng,Ickes,Flowers II,Gong
, p. 3574 - 3583 (2001)
Hydrogen-bonded molecular duplexes, 1·3 and 1·4, each of which contains a mismatched binding site (acceptor-to-acceptor in 1·3, and donor-to-donor in 1·4), were designed and synthesized based on duplex 1·2. One- and two-dimensional NMR studies demonstrated that, despite their single mismatched binding sites, the backbones of duplexes 1·3 and 1·4 still stayed in register through the formation of the remaining five H-bonds. The backbones of 1·3 and 1·4 adjusted to the presence of the mismatched binding sites by slightly twisting around these sites, which alleviate any headon repulsive interactions between two H-bond donors (amide O) or between two acceptors (amide H). After 1 equiv of single strand 2, which forms a perfectly matched duplex 1·2 with single strand 1, was added into the solution of either 1·3 or 1·4, only 1·2 and single strand 3 or 4, were detected. Isothermal titration calorimetry (ITC, in chloroform containing 5% DMSO) indicated that duplexes 1·3 and 1·4 were significantly (>40 times) less stable than the corresponding perfectly hydrogenbonded duplex 1·2. These NMR and ITC results indicate that the pairing of two complementary single strands is not affected by another very similar single strand that contains only one wrong H-bond donor or acceptor, which demonstrates that the self-assembly of this class of H-bonded duplexes is a highly sequence-specific process. The role of these H-bonded duplexes as predictable and programmable molecular recognition units for directing intermolecular interactions has thus been established.
