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7279-71-2

Bis(4-nitrophenyl)phosphate ion, also known as BNPP, is a chemical compound with the formula C12H6N2O11P. It is a yellow crystalline solid that is soluble in water and polar organic solvents. BNPP is a derivative of the phosphate ion, featuring two 4-nitrophenyl groups attached to the central phosphorus atom. bis(4-nitrophenyl)phosphate ion is commonly used as a substrate in enzyme assays, particularly for the enzyme alkaline phosphatase, due to its ability to undergo hydrolysis when acted upon by the enzyme. The resulting product, 4-nitrophenol, is a yellow compound that can be easily quantified, making BNPP a valuable tool for monitoring enzyme activity and studying enzyme kinetics.

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  • 7279-71-2 Structure

  • Basic information

    1. Product Name: bis(4-nitrophenyl)phosphate ion
    2. Synonyms: bis(4-nitrophenyl)phosphate ion
    3. CAS NO:7279-71-2
    4. Molecular Formula:
    5. Molecular Weight: 339.178
    6. EINECS: N/A
    7. Product Categories: N/A
    8. Mol File: 7279-71-2.mol

  • Chemical Properties

    1. Melting Point: N/A
    2. Boiling Point: N/A
    3. Flash Point: N/A
    4. Appearance: N/A
    5. Density: N/A
    6. Refractive Index: N/A
    7. Storage Temp.: N/A
    8. Solubility: N/A
    9. CAS DataBase Reference: bis(4-nitrophenyl)phosphate ion(CAS DataBase Reference)
    10. NIST Chemistry Reference: bis(4-nitrophenyl)phosphate ion(7279-71-2)
    11. EPA Substance Registry System: bis(4-nitrophenyl)phosphate ion(7279-71-2)

  • Safety Data

    1. Hazard Codes: N/A
    2. Statements: N/A
    3. Safety Statements: N/A
    4. WGK Germany:
    5. RTECS:
    6. HazardClass: N/A
    7. PackingGroup: N/A
    8. Hazardous Substances Data: 7279-71-2(Hazardous Substances Data)

7279-71-2 Usage

Check Digit Verification of cas no

The CAS Registry Mumber 7279-71-2 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 7,2,7 and 9 respectively; the second part has 2 digits, 7 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 7279-71:
(6*7)+(5*2)+(4*7)+(3*9)+(2*7)+(1*1)=122
122 % 10 = 2
So 7279-71-2 is a valid CAS Registry Number.

7279-71-2Relevant articles and documents

Dinuclear zinc complexes of phenol-based "end-off" compartmental ligands: Synthesis, structures and phosphatase-like activity

Abe,Izumi,Ohba,Yokoyama,Okawa

, p. 85 - 95 (2007/10/03)

The phenol-based compartmental ligands of the "end-off" type, 2,6-bis{N-[2-(dimethylamino)ethyl]iminomethyl})-4-methylphenol (HL1), 2-{N-[2-(dimethylamino)ethyl]iminomethyl}-6-{N-methyl-N-[2-(dimethylamino) ethyl]aminomethyl}-4-bromophenol (HL2), 2,6-bis{2-[(2-pyridyl)ethyl]iminomethyl}-4-methylphenol (HL3) and 2-[N,N-di(2-pyridylmethyl)aminomethyl]-6-{N-[2-(dimethylamino)ethyl] iminomethyl}-4-methylphenol (HL4), have formed dinuclear zinc complexes: [Zn2(L1)(AcO)2]PF6 (1), [Zn2(L1)(NCS)3] (2), [Zn2(L2)(AcO)2]PF6 (3), [Zn2(L2)(NCS)3] (4), [Zn2(L3)-(AcO)2]PF6 (5), [Zn2(L3)(NCS)3] (6), [Zn2(L4)(AcO)2]C104 (7), [Zn2(L4)(AcO)(NCS)2] (8) and [Zn2(L4)(NCS)3] (9). The crystal structures of 1, 5·(DMF)0.5(2-PrOH)0.5, 7·CHCl3, 8·(2-PrOH) and 9·3CHCl3 have been determined. Complexes 1 and 5·(DMF)0.5(2-PrOH)0.5 have a di-μ-acetato-μ-phenolato-dizinc(II) core comprised of two square-pyramidal Zn centers. 7·CHCl3 has a similar dinuclear core, but it is comprised of one square-pyramidal Zn and one pseudo-octahedral Zn. 8·(2-PrOH) has a μ-acetato-μ-phenolato-dizinc(II) core with a unidentate thiocyanato-N group on each Zn. 9·3CHCl3 exists in two different crystals: one has a μ-thiocyanato-N-μ-phenolato-dizinc(II) core, whereas the other has a μ-phenolato-dizinc(II) core. The diacetato complexes, 1, 3, 5 and 7, are stable in solution. Hydrolytic activities of the complexes toward tris(p-nitrophenyl) phosphate (TNP) have been studied in aqueous DMF by means of UV-visible spectroscopic and 31P NMR methods. Complexes 1, 3 and 5 have an activity to hydrolyze TNP into bis(p-nitrophenyl) hydrogenphosphate (HBNP) in aqueous DMF. In contrast, 7 showed little hydrolytic activity toward TNP in aqueous DMF.

Roles of zinc(II) ion in phosphatases. A model study with zinc(H)-macrocyclic polyamine complexes

Koike, Tohru,Klmura, Eiichi

, p. 8935 - 8941 (2007/10/02)

The zinc(II) complexes of 1,5,9-triazacyclododecane (L4, [12]aneN3) and 1,4,7,10-tetraazacydododecane (L2, [12]aneN4) promote the hydrolysis of tris(4-nitrophenyl) phosphate, TNP° (a neutral phosphotriester), an

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