76204-02-9 Usage
Description
4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid is a fluorogenic substrate of neuraminidases. 4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid is cleaved by neuraminidases to release the fluorescence moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases. 4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid has been used to quantify neuraminidase activity in mammalian cells and clinical isolates of influenza virus.
Chemical Properties
Off-White to Yellow Solid
Uses
Different sources of media describe the Uses of 76204-02-9 differently. You can refer to the following data:
1. Used as a substrate in a fluorometric assay of neuraminidase.
2. (4-Methylumbelliferyl)-N-acetyl-α-D-neuraminic acid sodium salt has been used for fluorometric assay of neuraminidase as well as fluorescent staining of sialidases in PAGE.
Biochem/physiol Actions
Substrate for fluorometric assay of neuraminidase. Used for fluorescent staining of sialidases in PAGE.
Check Digit Verification of cas no
The CAS Registry Mumber 76204-02-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 7,6,2,0 and 4 respectively; the second part has 2 digits, 0 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 76204-02:
(7*7)+(6*6)+(5*2)+(4*0)+(3*4)+(2*0)+(1*2)=109
109 % 10 = 9
So 76204-02-9 is a valid CAS Registry Number.
InChI:InChI=1/C21H25NO11.Na/c1-9-5-16(27)31-15-6-11(3-4-12(9)15)32-21(20(29)30)7-13(25)17(22-10(2)24)19(33-21)18(28)14(26)8-23;/h3-6,13-14,17-19,23,25-26,28H,7-8H2,1-2H3,(H,22,24)(H,29,30);/q;+1/p-1/t13?,14-,17?,18-,19?,21?;/m1./s1