78098-94-9Relevant academic research and scientific papers
Improved Synthesis of Oligodeoxyribonucleotides by Solid-Phase Phosphotriester Method Utilizing O6--2'-deoxyguanosine Derivatives
Chollet, Andre,Ayala, Edgar,Kawashima, Eric H.
, p. 1356 - 1364 (2007/10/02)
The synthesis of oligodeoxyribonucleotides on a cross-linked polystyrene solid support utilizing stable mono- and dinucleotide phosphotriester building blocks is presented.The use of O6--2'deoxyguanosine derivatives yields cleaner DNA fragments by supressing side reactions.Modifications improving the phosphotriester methodology are presented.The purification methods and analysis of synthetic oligodeoxyribonucleotides are described.
