78166-34-4Relevant academic research and scientific papers
CLINICAL ANALYSIS OF UREA NITROGEN. CHEMICAL STRUCTURE OF THE COLORED REACTION PRODUCT OF UREA WITH DIACETYLMONOXIME AND THIOSEMICARBAZIDE
Yuki, Hidetaka,Ohmura, Takeo,Kawasaki, Hideki,Yajima, Takehiko,Saito, Kimitoshi
, p. 1096 - 1100 (2007/10/02)
The chemical structure of the color reaction product, 2-amino-5,6-dimethyldihydro-3H-1,2,4-triazine-3-one (3a), obtained from the reaction of urea with diacetylmonoxime (DAM) and thiosemicarbazide (TSC) in 10percent sulfuric acid in methanol was elucidated.The ethyl and p-tolyl derivatives (3b and 3c) of 3a were also obtained from the reactions of ethyl- and p-tolylurea with DAM and TSC, along with 1,1'-disubstituted-5,4'-dimethyl-2,2'-dioxo-4,5'-diimidazolylmethanes. 3a and its derivatives, 3b and 3c, develop a red color immediately in acidic media and are thought to be the main products of the color reaction of urea, ethylurea and p-tolylurea, respectively, with DAM in the presence of TSC.A reaction mechanism is proposed.Keywords: urea; diacetylmonoxime; thiosemicarbazide; 2-amino-5,6-dimethyl-dihydro-3H-1,2,4-triazine-3-one; structural determination; diacetylmonoximethiosemicarbazone; urea derivatives
